基于不同电化学探针检测乳腺癌基因片段电化学传感器的研究  

Determination of Breast Cancer Genes Using an Electrochemical DNA Sensor Based on Three Different Probes

作　　者：贺彩梅 胡晓琴[1] 刘舒萍 李晓霞[1] HE Cai-mei;HU Xiao-qin;LIU Shu-ping;LI Xiao-xia(College of Chemistry and Chemical Engineering,Yan an University,Yan an 716000,China)

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《分析测试学报》2018年第3期269-274,共6页Journal of Instrumental Analysis

基　　金：延安大学科研项目(YD2014-04)

摘　　要：构建了以3种不同电活性物质(铁氰化钾平衡电对、亚甲基蓝和六氨合钌)为电化学信号探针,检测乳腺癌基因片段(乳腺癌DNA)的电化学传感器。利用吸附作用将探针ss DNA固定于金纳米-多壁碳纳米管-Nafion复合纳米材料修饰金电极表面,制备了DNA电化学传感器。采用循环伏安法、电化学阻抗法和微分脉冲伏安法,对DNA电化学传感器进行表征和定量分析。实验结果表明,在5 mmol/L K3[Fe(CN)6]-5mmol/L K4[Fe(CN)6]平衡电对电化学探针检测液中,乳腺癌DNA的线性范围为0.1~500.0 nmol/L,其检出限(S/N=3)为0.03 nmol/L。以20μmol/L亚甲基蓝为电化学探针检测液时,乳腺癌DNA的线性范围为1.0~500.0 nmol/L,检出限为0.3 nmol/L。利用50μmol/L六氨合钌电化学探针检测时,乳腺癌DNA的线性范围为1.0~500.0 nmol/L,检出限为0.3 nmol/L。3种电化学探针中,利用铁氰化钾平衡电对探针检测乳腺癌DNA的检出限最低,线性范围最宽。该传感器可用于其他DNA的检测分析。A novel DNA sensor was designed for the determination of breast cancer genes based on three electrochemical probes,the negative electrochemical probe[Fe(CN)6]3-/4-,methylene blue and the positive electrochemical probe[Ru(NH 3)6]3+.The breast cancer genes DNA sensor was fabricated by immobilizing a breast cancer gene ssDNA onto the surface of gold nanoparticles-multi-walled carbon nanotubes(MWCNT)-Nafion modified gold electrode through adsorption,and characterized by cyclic voltammetry,differential pulse voltammetry and electrochemical impedance method.Under the optimized conditions,in 5 mmol/L K 3[Fe(CN)6]-5 mmol/L K 4[Fe(CN)6](pH=7.4)solution,the electrochemical current response of the sensor was linear with the logarithm of the breast cancer gene concentration in range of 0.1-500.0 nmol/L,with a detection limit(S/N=3)of 0.03 nmol/L.While using methylene blue as the electrochemical probe,the electrochemical current response was linear with concentration of breast cancer gene in range of 1.0-500.0 nmol/L,and the detection limit was 0.3 nmol/L.Based on the different binding ability of[Ru(NH 3)6]3+with ssDNA and dsDNA,the current response of the sensor using[Ru(NH 3)6]3+as the probe was still linear with concentration of breast cancer gene in the range of 1.0-500.0 nmol/L.The detection limit was 0.3 nmol/L.Among three kinds of electrochemical probes,[Fe(CN)6]3-/4-probe had the lowest detection limit and the widest linear range for the detection of breast cancer gene.

关 键 词：电化学传感器 乳腺癌基因片段 电化学探针 多壁碳纳米管 金纳米 NAFION 

分 类 号：O657.1[理学—分析化学]