基于单壁碳纳米管阵列的克伦特罗无酶传感器研究  

Electrochemical Construction of Vertical Single-Walled Carbon Nanotubes Array as Clenbuterol Sensor

作　　者：刘佳 秦怡璠 朱洪 赵建庄 

机构地区：[1]北京农学院生物科学与工程学院,中华人民共和国农业部华北都市农业重点实验室,农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206

出　　处：《分析化学》2018年第2期225-231,共7页Chinese Journal of Analytical Chemistry

基　　金：本文系北京市教委科研计划项目(No.KM201610020002)及北京农学院科研项目(No.BJNXYTW20160412)资助

摘　　要：采用循环伏安法(CV)将单壁碳纳米管(SWCNTs)键合在玻碳电极上,制备单壁碳纳米管阵列(v-SWCNT)修饰电极。羧基化碳纳米管以乙二胺(EDA)为桥梁,形成酰胺键,使碳纳米管在玻碳电极(GCE)上有序稳定排布。制备的v-SWCNT电极稳定性好,对盐酸克伦特罗(CLE)的检测灵敏度高。实验结果表明,碳纳米管有规则的链接方式提高了利用效率。有序键合在电极表面的的碳纳米管,因其良好的加速电子转移作用、纳米催化效应及吸附作用,检测CLE的峰电流较GCE提高了一个数量级以上。CLE浓度在10~120 ng/m L范围内与电极的响应电流呈良好的线性关系。此电极用于人尿液中CLE的检测,结果令人满意。本研究制备的单壁碳纳米管阵列电极作为新型高灵敏CLE电化学无酶传感器,可望进一步开发用于实际临床检测。Vertical single-walled carbon nanotubes( v-SWCNTs) array was constructed on glassy carbon electrode( GCE) by electrochemical method of electro-cyclic voltammetry( CV method). The synthesized electrode was very stable and was not easy to fall off. Via the amino groups of ethylenediamine( Ethylenediamine,EDA) and the carboxyl group of carboxylated carbon nanotubes,the SWCNTs were ordered to grow steadily on GCE( v-SWCNTs/EDA/GCE). The modified electrode was used to detect hydrochloric acid clenbuterol( CLE). The experimental results showed that the regular link of carbon nanotubes on GCE improved its utilization efficiency. The detection sensitivity of clenbuterol was 16. 1 times higher than that of the bare GCE. Due to electron accelerating effect and nanometer effect of SWCNTs,the carboxyl peak current of SWCNTs was increased with the added CLE. The carboxyl peak current of SWCNTs had a good linear relationship with CLE concentration in the range of 10-120 ng/m L. The method was successfully applied to the determination of CLE in real urine samples with good recoveries. Also v-SWCNTs/EDA/GCE could be used as a new highly sensitive electrochemical sensor for CLE detection.

关 键 词：循环伏安法 盐酸克伦特罗 碳纳米管 单壁碳纳米管阵列 

分 类 号：O657.1[理学—分析化学]