三种金黄色葡萄球菌选择性分离培养基的检测效果比较  

Detection effects compared between three types of selective media for Staphylococcus aureus

作　　者：顾其芳[1] 张红芝[1] 朱颖莹[1] 鲁珺琰 於颖[1] 刘诚[1] 刘玥[1] 陈敏[1] GU Qi-fang;ZHANG Hong-zhi;ZHU Ying-ying;LU Jun-yan;YU Ying;LIU Cheng;LIU Yue;CHEN Min(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]上海市疾病预防控制中心,上海200336

出　　处：《上海预防医学》2018年第1期69-73,共5页Shanghai Journal of Preventive Medicine

基　　金：第四轮公共卫生三年行动计划重点学科传染病与卫生微生物学(15GWZK0101)

摘　　要：【目的】比较三种金黄色葡萄球菌选择性分离培养基的检测效果。【方法】分别使用BairdParker培养基(BP)、兔血浆纤维蛋白原培养基(RPF)和科玛嘉葡萄球菌显色培养基(CHROMagar^(TM) Staph aureus)对标准菌株、食品分离株以及食品样品进行对比检测。【结果】三种选择性培养基对金黄色葡萄球菌的回收率均达到95%以上;RPF和CHROMagar^(TM) Staph aureus培养基的检测效率明显高于BP培养基;且两种培养基敏感性、特异性均优于传统的BP培养基。【结论】应用RPF和CHROMagar^(TM) Staph aureus培养基检测食品中金黄色葡萄球菌,较传统的BP培养基具有更好的检测效果,建议将RPF和CHROMagar^(TM) Staph aureus培养基作为可靠的分离检测培养基应用于食品中金黄色葡萄球菌的分离检测。[Objective]To compare the detection Gfects between three kinds of selective media for Staphylococcus aureus.[Methods]Baird-Parker(BP)agar,Rabbit Plasma Fibrinogen(RPF)agar and CHROMagarTMStaph aureus were selected to test the standard strains,food-isolated strains as well as food samples.[Results]More than 9 5%recover efficiency could be reached by utilization of all the three types of media.However,the detection effects of RPF and CHROMagarTMStaph aureus were significantly higher than that of BP agar.Moreover,their sensitivity and specificity were also superior to that of conditional BP agar.[Conclusion]Better detection effect is obtained from RPF and CHROMagarTMStaph when compared to that from BP agar,suggesting that RPF and CHROMagar'rMStaph aureus agar are more reliable for the detection of Staphylococcus aureus in food.

关 键 词：金黄色葡萄球菌 Baird-Parker培养基 兔血浆纤维蛋白原培养基(RPF) 科玛嘉葡萄球菌显色培养基 检测效果 

分 类 号：R195[医药卫生—卫生统计学]