生物素化微细纤维肽结合IGF-Ⅰ对兔心肌细胞Akt蛋白表达及磷酸化的影响  

Effect of Biological microfiber peptides combine IGF-Ⅰon Akt expression and phosphorylation in Rabbit cardiomyocytes

作　　者：胡云华[1] 肖祖克[2] HU Yunhua;XIAO Zuke(Department of Cardiology,Jiangxi provincial People's Hospital,Nanchang,330006;Department of Respiratory Medicine,Jiangxi Provincial People's Hospital,Nanchang,330006,China)

机构地区：[1]江西省人民医院二部心内科,南昌330006 [2]江西省人民医院呼吸内科,南昌330006

出　　处：《江西医药》2018年第1期44-46,共3页Jiangxi Medical Journal

摘　　要：目的探讨生物素化微细纤维肽结合IGF-Ⅰ后对兔心肌细胞Akt蛋白表达及磷酸化的影响。方法体外培养胎兔心肌细胞,分别以生物素化微细纤维肽结合的IGF-Ⅰ和未结合的IGF-Ⅰ诱导Akt磷酸化,检测Akt、p-Akt蛋白表达,肌钙蛋白表达。结果与未结的IGF-Ⅰ相比,生物素化微细纤维肽结合的IGF-Ⅰ心肌细胞Akt、p-Akt、肌钙蛋白表达水平均显著升高(P<0.05)。结论生物素化微细纤维肽结合的IGF-Ⅰ不但保留了IGF-Ⅰ的生物蛋白活性,并且能加强和延长其作用,增强Akt、p-Akt、肌钙蛋白表达,增加蛋白质合成。Objective To study the effect of Biological microfiber peptides combine IGF-Ⅰon the Akt expression and phosphorylation in Rabbit cardiomyocytes.Methods Myocardial cells of rabbits were cultured in vitro,Induced Akt phosphorylation by IGF-Ⅰ,which is combined with biotin microfiber peptide and uncombined,the protein expression of Akt、p-Akt were observed after 24 hours.the protein expression of troponin were observed after 14 days.Results Comparing with uncombined IGF-Ⅰ,the protein expression of Akt,p-Akt troponin were significantly increased in IGF-Ⅰcombined with biotin microfiber peptide Myocardial cells(P<0.05).Conclusion The IGF-Ⅰcombined with biotin microfiber peptide,Not only the IGF-Ⅰbiological protein activity was preserved but also can be enhanced and extended,enhance protein expression of Akt,p-akt and troponin,Increase protein synthesis.

关 键 词：生物素化微细纤维肽 磷酸化 IGF-Ⅰ Akt、p-Akt 肌钙蛋白 

分 类 号：R54[医药卫生—心血管疾病]