白膜法手工制备血小板与机制血小板的质量分析  

Quality Analysis of Machine Preparation of Platelet and Manual Preparation of Buffer-coat-derived Platelet

作　　者：邓莉[1] 余利华[1] 杨冬燕[1] 邱本慧 马姗姗[1] 骆展鹏[1] 彭楷[1] 何大莉[1] DENG Li;YU Li-hua;YANG Dong-yan(Chongqing Blood Cencer Chongqing 400013)

机构地区：[1]重庆市血液中心,400013

出　　处：《临床输血与检验》2018年第1期18-20,共3页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：重庆市科委决策咨询与管理创新计划项目(No.cstc2016jccxBX0064)资助

摘　　要：目的比较手工分离及机器分离两种方法制备血小板的相关质量指标。方法对来源于街头采集的400 ml全血60袋,分别采用机器及手工分离白膜层,比较两种制备方法的白膜层血小板回收率、终产品血小板回收率、血小板含量、红细胞混入量、产品容量。结果机器分离血小板白膜层的血小板回收率及产品血小板回收率均高于手工分离制备(P<0.05)。两种制备方法的红细胞混入量的差异无统计学意义(P>0.05)。手工分离血小板终产品容量变异系数高于机器制备血小板(P<0.05)。结论机器制备浓缩血小板所收集的血小板回收率及容量均优于手工制备血小板。Ob jective Comparison of platelet quality between machine preparation of platelet and manual preparation.Method 400 ml of blood extracted from 60 donors.Platelet concentrates were obtained by methods of Machine preparation and manual preparation.Platelet recovery,platelet count,platelet capacity,red blood cell residue were detected.Result The Buffer-coat-derived platelet recovery rate by Machine were higher than by manual separation preparation(P<0.05).There is no difference of The account of Red blood cell residue by Machine and by manual(P>0.05).CV of platelet capacity by mannual was higher than that of machine(P<0.05).Conclusion Machine preparation of collected concentrated platelet has higher platelet recovery and capacity than manual preparation of platelets.

关 键 词：白膜法 手工血小板 机制血小板 质量分析 

分 类 号：R331.143[医药卫生—人体生理学]