血液筛查中1例低浓度HIV窗口期标本的检测  

Blood Screening Detected One Case of HIV Window Period of Low Concentration Samples

作　　者：金一鸣[1] 方志红[2] 陆荣[1] JIN Yi-ming;FANG Zhihong;LU Rong(Suzhou City Blood Center,Suzhou 215004)

机构地区：[1]江苏省苏州市中心血站,215004 [2]苏州市中医医院

出　　处：《临床输血与检验》2018年第1期42-44,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的采用血清学和分子生物学方法,确认1例低浓度HIV RNA窗口期献血者的感染状态。方法对血清学ELISA检测结果合格的献血者标本进行核酸检测,发现1例低浓度HIV窗口期标本,利用定性、定量核酸检测(NAT)方法,对该献血者不同时期的血液标本进行HIV病毒载量检测。结果献血者首次献血标本检测结果为血清学ELISA检测阴性,核酸定性试验为反应性,随后进行的核酸定量试验小于20拷贝/ml;对随访后采集的标本进行血清学和核酸检测,最终确认为血清学转阳。结论该献血者是1例病毒载量极低的HIV窗口期献血者,NAT与ELISA检测结合能进一步保障临床用血的安全。Objective Infection status one case of low concentrations of HIV RNA window period blood donor was confirmed by combined serological and molecular biological methods.Methods On serological ELISA results qualified blood specimens for nucleic acid detection,1 case of low concentration of HIV window period were found via the method of qualitative and quantitative detection of nucleic acids(NAT),the donor's samples in different periods of blood specimens of HIV viral load were detected.Results The first time blood donor samples tested negative by ELISA serology,nucleic acid reactive qualitative test,followed by quantitative nucleic acid testing is less than 20 copies/mL;for follow-up samples collected serological and nucleic acid testing,final confirmation serum turn positive.Conclution The blood donor is a very low viral load of 1 HIV window period of blood donors,NAT detection technology and ELISA detection combined to further protect the safety of clinical use of blood.

关 键 词：HIV 窗口期 ELISA 核酸检测 

分 类 号：R446.61[医药卫生—诊断学] R392.11[医药卫生—临床医学]