云南丘北仙女虾一物种的分子鉴定  

Molecular identification of fairy shrimp from Qiubei County,Yunnan Province

作　　者：李蓉[1] 陈艳艳[1] 和忠廷 杨洪福 李春青[1] 曾本娟 陈善元[1] 肖蘅[1] LI Rong;CHEN Yan-yan;HE Zhong-ting;YANG Hong-fu;LI Chun-qing;ZENG Ben-juan;CHEN Shan-yuan;XIAO Heng(School of Life Sciences,Yunnan University,Kunming 650091;Fisheries Administration of Qiubei County,Yunnan Province,Qiubei 663200,China)

机构地区：[1]云南大学生命科学学院,昆明650091 [2]云南省丘北县渔业站,丘北663200

出　　处：《生物学杂志》2018年第1期46-51,共6页Journal of Biology

基　　金：云南大学引进人才科研启动资金;云南省海外高层次引进人才计划项目

摘　　要：为了从分子水平上明确云南丘北仙女虾一物种的具体种类,采用PCR产物直接测序法测定了30个样本的COI基因与18S r DNA基因的部分序列片段(长度分别为658 bp和325 bp)。基于NCBI数据库BLAST相似性比对发现COI基因与18S r DNA基因序列同Streptocephalus sirindhornae相似度最高,分别为96%和100%。序列分析发现COI基因序列中共检测到15个简约信息位点,22个变异位点,8个单倍型;18S r DNA基因序列中只检测到1个简约信息位点,1个变异位点,2个单倍型。此外,结合Gen Bank中无背甲目部分科物种的同源序列,进行遗传距离和系统发育关系分析。结果显示:丘北仙女虾与弯头虫科的S.sirindhornae种间遗传距离为4.3%,属于种内水平;与钗额虫科的Thamnocephalus platyurus种间遗传距离为21.1%,属于种间水平。此外,系统发育树显示:丘北仙女虾与S.sirindhornae聚在一起形成一个单系枝,COI树的支持率为100%,18S r DNA树的支持率为72%。上述结果表明云南丘北仙女虾是弯头虫科的Streptocephalus sirindhornae,而不是钗额虫科的物种。同时,还表明COI基因相比18S r DNA基因更适合用于仙女虾物种的分子鉴定。In order to clarify the taxonomic status of fairy shrimps from Qiubei County of Yunnan Province at the molecular level,this study applied PCR amplification and sequencing methods to determine the COI gene( 658 bp) and 18 S r DNA gene( 325 bp) sequences of 30 fairy shrimp samples. The BLAST similarity analyses based on NCBI database showed that COI gene and 18 S r DNA gene sequences were highly similar to those of Streptocephalus sirindhornae,with identity values of 96% and 100%,respectively. Sequence analyses revealed that there were 15 parsimony informative sites and 22 variable sites that defined 8 haplotypes in the COI gene sequences and there was one parsimony informative site and one variable site that defined two haplotypes in 18 S r DNA gene sequences. In addition,the genetic distances and phylogenetic relationships were analyzed based on the combined dataset including our sequence data and homologous COI sequences of some Anostraca families in Gen Bank. The results showed that: interspecific average genetic distance between fairy shrimps from Qiubei and Streptocephalus sirindhornae of family Streptocephalidae was 4. 3%,being at intraspecific level;while interspecific average genetic distance between fairy shrimps from Qiubei and Thamnocephalus platyurus of family Thamnocephalidae was 21. 1%,being at interspecific level. In addition,phylogenetic trees showed that fairy shrimps from Qiubei and Streptocephalus sirindhornae clustered together as a monophyletic clade,with support values of 100% and 72% on COI and 18 S r DNA trees,respectively. These results mentioned-above indicated that the fairy shrimp species from Qiubei County of Yunnan Province was Streptocephalus sirindhornae of family Streptocephalidae,but was not species of family Thamnocephalidae. At the same time,it was suggested that COI gene is a more suitable marker than 18 S r DNA gene for molecular identification of fairy shrimps.

关 键 词：仙女虾 COI基因 18S RDNA基因 分子鉴定 

分 类 号：Q953[生物学—动物学]