不同地理种群中缅树鼩MC1R基因的差异性研究  

Research on the differences between different geographic populations of tree shrew (Tupaia belangeri ) Mc1r gene

作　　者：付家豪 何一帆 朱万龙[1] 高文荣[1] 王政昆[1] FU Jia-hao;HE Yi-fan;ZHU Wan-long;GAO Wen-rong;WANG Zheng-kun(Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of University in Yunnan Province,School of Life Science,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室云南师范大学生命科学学院,昆明650500

出　　处：《生物学杂志》2018年第1期52-55,59,共5页Journal of Biology

基　　金：国家自然科学基金项目(31660121);云南省应用基础研究计划重点项目(2016FA045);云南省科技计划项目(2015GA008)

摘　　要：黑色素皮质激素受体1基因(Melanocortin receptor 1,Mc1r)作为控制动物毛色的主效候选基因,在动物的毛色中发挥了重要作用。以10个地理种群112只中缅树鼩为研究对象,采用PCR扩增测序共获得954 bp mc1r序列。结果显示:1)954 bp mc1r序列共编码317个氨基酸,蛋白质二级结构以α-螺旋为主,并含有7个跨膜结构域。2)mc1r构建的系统进化树结果显示,10个地理种群中缅树鼩聚为4支,海南种群一支、大新种群一支、片马种群一支、其他种群一支。结果说明,不同地理种群中缅树鼩生存环境的差异导致了毛色的差异。Melanocortin receptor 1 gene(Melanocortin Receptor 1,Mc1r)is the major candidate gene in the regulation of mammalian coat color,which plays an important role in animal′s coat color.There were 112 Tupaia belangeri collected from 10 geographical populations,and 954 bp mc1r sequence was amplified by PCR and analyzed.The results showed that the 954 bp mc1r sequence encoded 317 amino acids.The secondary structure of protein was mainly composed ofα-helix containing 7 transmembrane domains.Phylogenetic tree of mc1r showed that 10 populations of tree shrews were clustered into 4 branches:CladeⅠ(HaiNan Population),CladeⅡ(DaXin Population),CladeⅢ(PianMa Population)and CladeⅣ(Dali,Mengla,Hekou,Kunming,Leye,Xingyi and Xichang populations).The results showed that different living environments cause the differences in tree shrews’s coat color

关 键 词：黑色素皮质激素受体1基因 中缅树鼩 地理种群 

分 类 号：Q78[生物学—分子生物学]