冷驯化下中缅树鼩脂肪细胞的流式分析  

Flow cytometric analysis of fat cells in Tupaia belangeri under cold acclimation

作　　者：朱万龙[1] 侯东敏[1] 孙舒然[1] 王政昆[1] ZHU Wan-long;HOU Dong-min;SUN Shu-ran;WANG Zheng-kun(Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室云南师范大学生命科学学院,昆明650500

出　　处：《生物学杂志》2018年第1期60-63,共4页Journal of Biology

基　　金：国家自然科学基金项目(31660121);联合支持国家计划项目(2015GA008);云南省科技计划重点项目(2016FA045)

摘　　要：首次成功探索出了利用中缅树鼩脂肪组织流式样品的制作方法,首次利用流式细胞术对冷驯化下中缅树鼩脂肪细胞进行对比分析。结果表明:冷驯化28 d时中缅树鼩腹部大网膜、腹股沟等白色脂肪组织的单一R1群中有5.70%的细胞显示为UCP1阳性。至49 d时白色脂肪组织中出现了新的细胞群R2群。其中R1群有69.64%的细胞显示为UCP1阳性,R2有95.28%的细胞显示为UCP1阳性。冷驯化使白色脂肪组织中出现UCP1表达阳性的细胞群。以上结果说明冷驯化不仅使中缅树鼩BAT组织增生、产热活性增强,还诱导了其白色脂肪组织中米色脂肪细胞的出现。In the present study,it was first successful using of adipose tissue making method of flow in Tupaia belangeri,and the flow cytometry was also firstly used to compare and analyze fat cells under cold acclimation.The results showed that 5.70%of white adipose tissue(WAT)in abdominal omental and groin had single R1 group and showed positive UCP1 on 28 d of cold acclimation.New cell population R2 group appeared in WAT in the 49 th day,including 69.64%the cells in R1 group of showing positive for UCP1.95.28%of the cells in R2 had showing positive for UCP1.Cold acclimation makes UCP1 expression positive cell population in WAT.All of the results suggested that cold acclimation not only made the brown adipose tissue hyperplasia,the production of thermal activity increased,but also induced the beige fat cells appeared in WAT.

关 键 词：中缅树鼩 脂肪组织 流式细胞术 

分 类 号：Q955[生物学—动物学]