分子标记辅助选择改良C815S的稻瘟病抗性  

Improvement of rice blast resistance of C815S through molecular marker–assisted selection

作　　者：向聪[1,2] 任西明 雷东阳[1,2] 陈英 XIANG Cong;REN Ximing;LEI Dongyang;CHEN Ying(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]水稻油菜抗病育种湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2018年第1期62-65,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省教育厅项目(15B117);长沙市科技计划项目(kq1706022);水稻油菜抗病育种湖南省重点实验室开放课题(SYKB201706)

摘　　要：为提高水稻两系不育系C815S的稻瘟病抗性,以含广谱抗稻瘟病基因Pigm的谷梅4号为供体,C815S为受体,通过杂交、回交并结合分子标记辅助选择,将Pigm基因导入C815S中,经抗性鉴定和综合农艺性状评价,获得了3个携带纯合抗性基因的改良不育株系(7CS01、7CS02、7CS03)。抗性鉴定结果显示:7CS01病级为1级,表现为抗;7CS02和7CS03病级为3级,表现为中抗;改良不育株系稻瘟病抗性综合指数平均较C815S提高58.05%;改良不育系穗数平均比C815S提高26.45%。To improve the blast resistance of C815S,a widely applicable two–line photo–thermal–sensitive male sterile in China,Gumei 4 contained rice blast risistance gene Pigm was used as gene donor to introduce Pigm into the genetic background of C815S by cross and then back cross breeding combined with molecular marker–assisted selection(MAS).Three improved sterile lines with Pigm homozygous genotype were developed through blast resistance identification and evaluation of comprehensive agronomic characters.7CS01 showed 1st grade for blast,meant resistance;7CS02 and 7CS03 showed 3rd grade for blast,meant middle resistance.Blast resistance comprehensive index of the improved sterile lines was raised by 58.05%and average spike number raised by 26.45%,compared to C815S.

关 键 词：稻瘟病抗性 水稻两系不育系C815S 分子标记辅助选择 抗性鉴定 

分 类 号：S511.035.4[农业科学—作物学]