人工感染细粒棘球蚴犬粪中蛋白质组的分析  

Proteomic Analysis of Echinococcus granulosus Tapeworm in Dog Feces Post Artificial Infection

作　　者：李双男 闫鸿斌[1] 李立[1] 姚刚 田文俊 李文卉[1] 张念章[1] 吴燕涛 付宝权[1,2] 贾万忠 LI Shuang-nan;YAN Hong-bin;LI Li;YAO Gang;TIAN Wen-jun;LI Wen-hui;ZHANG Nian-zhang;WU Yan-tao;FU Bao-quan;JIA Wan-zhong(Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Zoonosis of Agriculture Ministry/ State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,CAAS,Lanzhou,Gansu,730046,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease,Yangzhou,Zhejiang,225009,China)

机构地区：[1]中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/农业部兽医公共卫生重点实验室/甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《动物医学进展》2018年第1期7-15,共9页Progress In Veterinary Medicine

基　　金：国家重点研发计划项目(2017YFD0501301);国家自然科学基金项目(31402191;31401148)

摘　　要：收集感染细粒棘绦虫原头蚴后不同时间的犬粪,分离和提取蛋白质,对蛋白质在胶内酶切后,依次进行质谱分析、生物信息学和蛋白质功能富集分析。结果发现,在犬粪中共鉴定出蛋白质3 242个,其中宿主(犬)蛋白有3 107个、细粒棘球绦虫蛋白有135个。蛋白质功能富集结果显示,犬感染细粒棘球绦虫后,寄生虫与宿主相互作用,虫体在不同时间分泌特定蛋白,而蛋白质的分子生物学功能存在一定差异。研究结果对建立粪抗原诊断方法和研究细粒棘球绦虫感染宿主后的代谢途径及其生活机制提供了重要依据。Proteins were extracted from the dog feces at different time post-infection(PI)with E.granulosus protoscoleces,digested with the enzymes,and subjected to electrospray ionization followed by tandem mass spectrometry(MS/MS),bioinformatics and protein function analysis.In total,3 242 proteins were identified in the dog feces,of which 3 107 were from the host,and 135 were from the E.granulosus tapeworm.At different time PI,interactions appeared between the host and the parasite E.granulosus.From the results,different specific proteins were secreted by the parasite,and their different biological functions were manifested.This study may provide foundation for establishing new diagnostic methods based-on copro-antigens and for exploring the life mechanisms of the E.granulosus during the different periods PI.

关 键 词：细粒棘球绦虫 犬粪 不同时间 蛋白质组 生物信息学分析 

分 类 号：S852.734[农业科学—基础兽医学] S858.292[农业科学—兽医学]