不同CpG ONDs对猪源细胞免疫分子转录水平的影响  

Effects of Different CpG Oligodeoxynucleotides on Transcriptions of Immune Related Molecules in Porcine Cells

作　　者：邓慧 张丽琳 黎瑞巧 张蕾 黄金海 DENG Hui;ZHANG Li-lin;LI Rui-qiao;ZHANG Lei;HUANG Jin-hai(School of Life Sciences,Tianjin University,Tianjin,300072,China)

机构地区：[1]天津大学生命科学学院,天津300072

出　　处：《动物医学进展》2018年第1期22-30,共9页Progress In Veterinary Medicine

基　　金：天津市应用基础及前沿技术研究计划重点项目(17JCZDJC33900)

摘　　要：为了探究不同CpG ODNs对猪免疫调节作用,设计合成了7条包含CpG单元的寡聚核苷酸。通过体外刺激3种猪源细胞,包括猪小肠上皮细胞(IPEC-J2)、猪肺泡巨噬细胞(3D4/21)和猪外周血单核细胞(PBMCs),用荧光定量PCR检测细胞因子转录水平的变化。同时探究了不同CpG ODNs处理过的IPEC-J2的抗病毒能力。不同CpG ODNs都能够使种猪源细胞TLR9和TLR3相关信号通路分子的转录水平升高,促进多种Th类型细胞因子转录水平的升高,促进干扰素和TNF-α和与抗原递呈相关分子转录水平升高。CpG ODNs能够协助细胞抵抗猪流行性腹泻病毒和水疱性口炎病毒的感染,但抗病毒能力存在差异。CpG ODNs可作为一种良好的猪用疫苗佐剂。Seven oligodeoxynucleotides containing CpG motif were designed to evaluate the immune-regulate effects of different CpG ONDs on pig cells.Three kinds of cells were treated by those CpG ODNs,and then the real-time PCR was used to detect the transcription level changes of some immune related molecules.This study also detected the antiviral activity of the IPEC-J2 cells treated with CpG ODNs.The result showed that the CpG ODNs could elevate the transcriptional levels of TLR9 and/or TLR3,the signaling transduction pathways molecules in porcine cells,and promote the transcription of various Th-type cytokines,interferon,TNF-αand gene related to antigen presentation.All CpG ODNs treated cells have significant antiviral effect both PEDV-or VSV-inoculated treated cells.The CpG ODNs can be used as efficient vaccine adjuvant in swine.

关 键 词：CPG寡聚脱氧核苷酸 猪 TOLL样受体 

分 类 号：S852.42[农业科学—基础兽医学]