禽呼肠病毒在鸡胚成纤维细胞系中的增殖规律  

Proliferation of Avian Reovirus in Chicken Embryo Fibroblast Cells

作　　者：沈文康 谢芝勋 王盛[1,2] 黄莉 谢丽基 黄娇玲 曾婷婷 张艳芳 张民秀 罗思思 范晴 谢志勤 邓显文 SHEN Wen-kang;XIE Zhi-xun;WANG Sheng;HUANG Li;XIE Li-ji;HUANG Jiao-ling;ZENG Ting-ting;ZHANG Yan-fang;ZHANG Min-xiu;LUO Si-si;FAN Qing;XIE Zhi-qin;DENG Xian-wen(College Of Animals Science and Technology,Guangxi University,Nanning,Guangxi,530004,China;Guangxi Key Laboratory of Veterinary Biological Technology,Guangxi Veterinary Research Institute,Nanning,Guangxi,530001,China)

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西壮族自治区兽医研究所/广西兽医生物技术重点实验室,广西南宁530001

出　　处：《动物医学进展》2018年第1期30-33,共4页Progress In Veterinary Medicine

基　　金：广西自然科学基金项目(2017GXNSFBA198140);国家自然科学基金项目(31660715);广西水产畜牧兽医局科技项目(桂渔牧科201452003);广西兽医生物技术室开放基金项目(14-045-31-A-2);国家"万人计划"领军人才专项项目(2016-37);中国东盟及一带一路南亚国家动物疫病防控技术研发联合实验室建设项目(桂科AB16380003)

摘　　要：为研究禽呼肠病毒(ARV)在鸡胚成纤维细胞(DF1)上的增殖规律,将ARV S1133株接种至DF1细胞,连续传代3次后观察细胞病变(CPE),RT-PCR检测病毒核酸。结果显示,ARV S1133株对DF1细胞有很好的亲嗜性,并出现典型的CPE。按照Reed-Meunch方法测量7个时间点的TCID50,并绘制生长曲线。结果显示,ARV感染DF1细胞后的0h^12h为静止期,12h^48h为快速增长期,并在48h达到最大的病毒效价,TCID_(50)为10^(-8.9)/0.1mL,为进一步研究ARV的致病机理,以及在DF1上研制禽呼肠病毒细胞灭活苗提供参考。To study the proliferation of avian reovirus(ARV)on chicken embryo fibroblasts(DF1),DF1 cells was infected with ARV S1133 strain and serialized continuouly for three passages.The cytopathic effect(CPE)was observed after virus infection,and virus was identified by RT-PCR and the proliferation of the virus was studied by Reed-Meunch method.The results showed that ARV S1133 strain was palpable to DF1 cells and had typical CPE.TCID 50 was measured at seven time points according to the Reed-Meunch method and plotted as a growth curve,the results revealed that ARV began to proliferate at hour 12,and reached a rapid proliferation during hour 12 to 48 post-infection.The virus titer reached the peak at hour 48,TCID 50 was 10-8.9/0.1 mL.This study provided a good platform for further study on the pathogenesis of ARV and a theoretical basis for the development of cell-inactivated vaccine of avian reovirus at DF1 cells.

关 键 词：禽呼肠病毒 鸡胚成纤维细胞 病毒效价 一步生长曲线 

分 类 号：S852.659.4[农业科学—基础兽医学]