FGF4在牛早期胚胎发育过程中的表达  

Expression of FGF4 in Bovine Embryos at Early Developmental Stage

作　　者：刘瑞琪[1] 王玮玮[1] 屈鹏祥 马晓楠 王勇胜[1] 卿素珠[1] LIU Rui-qi;WANG Wei-wei;QU Peng-xiang;MA Xiao-nan;WANG Yong-sheng;QING Su-zhu(College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China)

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《动物医学进展》2018年第1期56-60,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31472094)

摘　　要：应用实时荧光定量PCR和免疫荧光染色技术对成纤维生长因子4(FGF4)在牛早期胚胎发育各个阶段的表达和定位规律进行研究。结果表明,FGF4mRNA及蛋白在牛MⅡ期卵母细胞及各阶段早期胚胎均有表达,其表达量由强到弱依次为桑椹胚、囊胚、8-细胞胚、MⅡ期卵母细胞、4-细胞胚、2-细胞胚;从定位来看,FGF4在MⅡ期卵母细胞及8-细胞期前的各阶段早期胚胎的细胞质和细胞核中均有表达,而8-细胞期及之后的桑椹胚和囊胚期仅分布于细胞质。结果提示,FGF4在牛的早期胚胎发育过程中可能发挥重要作用。The expression and localization of FGF4 in bovine embryos at early developmental stages were studied by quantitative real-time PCR(qRT-PCR)and immunofluorescent staining.The results showed that FGF4 were detected in MⅡoocytes and all early embryos.The qRT-PCR results showed that the expressional intensities of FGF4 were morula,blastocyst,8-cell stage embryo,MⅡoocytes,4-cell stage embryo,2-cell stage embryo in descend order.The location of FGF4 was mainly on the nucleus and the cytoplasm at MⅡoocytes,2-cell stage and 4-cell stage embryos,but only on cytoplasm at 8-cell stage embryo,morula and blastocyst.The results indicated that FGF4 might have important roles in early embryonic development in bovine.

关 键 词：成纤维生长因子4 牛 早期胚胎 表达 

分 类 号：S852.16[农业科学—基础兽医学]