异叶青兰总黄酮对血管紧张素Ⅱ诱导的大鼠心肌细胞肥大的抑制作用  

Study on the effect of the total flavonoids of Dracocephalum Heterophyllum Benth Flavonoid on rat hypertrophy myocardial cell induced by angiotensin Ⅱ

作　　者：蒋红[1] 何雯[2] 张晨[1] JIANG Hong;HE Wen;ZHANG Chen(Xinjiang Medical University;The Fifth Affiliated Hospital,Xinjiang Medical University, Urumqi 830011,China)

机构地区：[1]新疆医科大学 [2]新疆医科大学第五附属医院,乌鲁木齐830011

出　　处：《新疆医科大学学报》2018年第2期253-257,261,共6页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81560689);新疆医科大学研究生创新创业项目(CXCY2017061)

摘　　要：目的观察异叶青兰总黄酮(Dracocephalum Heterophyllum Benth Flavonoid,DHBF)对血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)诱导的心肌细胞肥大的抑制作用,为进一步探讨其作用机制提供依据。方法 SD大鼠,0~3d龄,体外培养大鼠乳鼠的心肌细胞,实验分为对照组、AngⅡ(1μmol/L)组、缬沙坦(50μmol/L)+AngⅡ(1μmol/L)组、不同浓度的DHBF(10、25、50μmol/L)+AngⅡ(1μmol/L)组。用AngⅡ(1μmol/L)诱导心肌细胞肥大,DHBF和缬沙坦分别对其进行干预,CCK-8法观察心肌细胞活性,RT-PCR技术检测左心室原癌基因c-jun、心肌肥大基因心房利钠肽(ANP)、脑钠肽(BNP)的mRNA的表达水平,其内参因子为甘油醛-3-磷酸脱氢酶(GAPDH);激光共聚焦法检测心肌细胞的表面积。结果 (1)各组间细胞生存率差异有统计学意义(P<0.01),DHBF(50μmol/L)组细胞生存率明显高于其他各组(P<0.05);(2)各组间肥大标志物基因c-jun、ANP和BNP的mRNA表达差异有统计学意义(P<0.05),DHBF(50μmol/L)组的表达水平明显低于其他各组(P<0.05);(3)各组间肥大心肌细胞表面积差异有统计学意义(P<0.05),DHBF(50μmol/L)组的细胞表面积明显小于其他各组(P<0.05)。结论 DHBF能改善AngⅡ诱导的心肌细胞肥大的生存率,下调原癌基因c-jun和心肌肥大因子ANP、BNP的mRNA表达,减小由AngⅡ诱导的肥大心肌细胞的表面积。ObjiectiveEffect of Dracocephalum heterophyllum flavonoids(DHBF)of Uygur Medicine on the hypertrophy of rat cardiomyocyte,which were induced by on angiotensinⅡ(AngⅡ),and to provide a basis for further study of the mechanism.MethodsNeonatal rat myocardial cell(0-3 days of age)was cultured in vitro.The experiment was divided into control group,AngⅡ(1μmol/L)group,Valsartan(50μmol/L)+AngⅡ(1μmol/L)group,different concentrations of DHBF(10,25,50μmol/L)+AngⅡ(1μmol/L)group.hypertrophy cardiomyocyte were induced by AngⅡ1μmol/L,DHBF and valsartan respectively.The activity of myocardial cells was detected by CCK-8 method.The mRNA expression of c-jun gene,cardiac hypertrophy gene atrial natriuretic peptide(ANP)and brain natriuretic peptide(BNP)were dectected by qPCR and use glyceraldehyde-3-phosphate dehydrogenase(GAPDH)as a control gene.The surface area of myocardial cell was detected by confocal laser scanning.Result(1)In terms of myocardial cell activity,there was significant difference between the groups(P<0.01),the activity of DHBF(50μmol/L)group was significantly higher than the other groups(P<0.01);(2)Aspects of m RNA expression in c-jun,ANP,BNP:there was significant difference among the groups(P<0.01),the expression level of DHBF(50μmol/L)group was significantly lower than that of other groups(P<0.01);(3)In terms of cell surface area:there was significant difference among the groups,the cell surface area of DHBF(50μmol/L)group was significantly lower than the other groups(P<0.01).ConclusionThe activity of hypertrophy cardiomyocytes which were induced by AngⅡcan be improved,the mRNA expression of proto oncogene c-jun,ANP and BNP was down-regulated,and surface area of cardiomyocytes induced by AngⅡcould be reduced by DHBF,respectively.

关 键 词：心肌细胞 肥大 异叶青兰总黄酮 血管紧张素Ⅱ 

分 类 号：R29[医药卫生—民族医学] R363[医药卫生—中医学]