苦碟子注射液对大鼠肾脏缺血再灌注损伤的影响  

Effect of Kudiezi injection on renal ischemia-reperfusion injury in rats

作　　者：杨洁 董建 李运 史国辉 Jie Yang;Jian Dong;Yun Li;Guo-hui Shi(Department of Nephrology,the Affiliated Hospital,North China University of Science and Technology,Tangshan,Hebei 063000,China;Department of Nursing and Rehabilitation,North China University of Science and Technology,Tangshan,Hebei 063000,China)

机构地区：[1]华北理工大学附属医院肾内科,河北唐山063000 [2]华北理工大学护理与康复学院,河北唐山063000

出　　处：《中国现代医学杂志》2018年第4期6-10,共5页China Journal of Modern Medicine

摘　　要：目的观察苦碟子注射液对大鼠肾脏缺血再灌注损伤的影响并探讨其可能机制。方法将18只健康雄性SD大鼠随机分为假手术组(Sham组)、模型组(IRI组)、苦碟子注射液组(KDZ组)。采用全自动生化分析仪检测血清肌酐(Scr)、尿素氮(BUN),采用检测试剂盒分别检测肾组织中超氧化物歧化酶(SOD)、丙二醛(MDA)活力,HE染色观察病理组织学变化,免疫组织化学法观察肾组织中白细胞介素6(IL-6)表达情况,ELISA检测肾组织细胞间黏附分子-1(ICAM-1)的含量。结果与Sham组相比,IRI组Scr及BUN含量和肾组织MDA含量均升高,KDZ组Scr及BUN含量和肾组织MDA含量均介于Sham组与IRI组之间;IRI组肾组织SOD活性较Sham组降低,KDZ组SOD活性介于Sham组与IRI组之间。HE染色发现Sham组肾小管形态结构基本正常,管腔内仅有极少量上皮细胞脱落,间质内可见少量炎症细胞浸润;IRI组肾小管上皮细胞出现空泡变性,管腔缩小甚至堵塞,其内可见脱落细胞和管型,间质水肿明显,其内可见大量炎症细胞浸润;KDZ组肾小管上皮细胞变性不明显,管腔内可见极少量上皮细胞和管型,间质轻度水肿,可见少量炎症细胞浸润,KDZ组病理损伤程度轻于IRI组。免疫组织化学发现Sham组有少量IL-6表达,IRI组IL-6表达量较Sham组增多,KDZ组表达量较IRI组减少。ELISA检测发现IRI组、KDZ组ICAM-1含量均较Sham组增高,但KDZ组低于IRI组。结论苦碟子注射液能够减轻大鼠缺血再灌注损伤,其机制与其抗氧化应激、减轻炎症损伤有关。Objective To observe the effect of Kudiezi injection on renal ischemia-reperfusion injury in rats and to investigate its possible mechanisms.Methods A total of 18 male Sprasue-Dawley(SD)rats were randomly divided into three groups:sham group,model group(IRI group),and Kudiezi injection group(KDZ group).Serum creatinine(Scr)and blood urea nitrogen(BUN)were detected by automatic biochemistry analyzer.The activity of superoxide dismutase(SOD)and malondialdehyde(MDA)in the renal tissue was respectively assessed by relevant detection kits.Histopathological changes were observed after HE staining.The expression of interleukin 6(IL-6)in the renal tissue was checked by immunohistochemistry.The content of intercellular adhesion molecule-1(ICAM-1)was tested by ELISA.Results The levels of Scr,BUN and MDA in the renal tissue of the IRI group were significantly increased compared with the sham group,and the levels of Scr and BUN and MDA of the renal tssue in the KDZ group were between those in the sham group and the IRI group.The activity of SOD in the renal tissue of the IRI group was significantly lower than that of the sham group,while the activity of SOD in the renal tissue of the KDZ group was between that of the sham group and the IRI group.HE staining showed that the morphology and structure of renal tubules in the sham group were basically normal,only a very small number of shedding epithelial cells were observed in the lumen and a small amount of inflammatory cell infiltration was noted in the stroma.In the IRI group,vacuolar degeneration of renal tubular epithelial cells was seen,the tubular lumen became narrow even blocked,there were shedding cells and tubular casts in the lumen and evident stromal edema as well as obvious inflammatory cell infiltration in the stroma.In the KDZ group,degeneration of renal tubular epithelial cells was not apperent,a very small quantity of epithelial cells and tubular casts were noted in the lumen,slight stromal edema and slight inflammatory cell infiltration in the stroma were obser

关 键 词：苦碟子注射液 肾脏 缺血再灌注损伤 ICAM-1 大鼠 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学]