GBE对COPD大鼠血清和肺泡灌洗液中IL-1、IL-8含量的影响  

Effect of Ginkgo biloba extract on content of IL-1 and IL-8 in serum and bronchial alveolar lavage fluid in rat model of COPD

作　　者：谭玉萍[1] 张鹏飞[1] 冯玉清 杨益宝[1] 梁炜[1] 杨红梅[1] 梁爱武[1] 邓祯[1] Yu-ping Tan;Yu-ping Tan;Yu-qing Feng;Yi-bao Yang;Wei Liang;Hong-mei Yang;Ai-wu Liang;Zhen Deng(Department of Respiratory Medicine,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi 530001,China;Clinical Laboratory,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi 530001,China)

机构地区：[1]广西中医药大学附属瑞康医院呼吸内科,广西南宁530001 [2]广西中医药大学附属瑞康医院检验科,广西南宁530001

出　　处：《中国现代医学杂志》2018年第5期7-10,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81260005)

摘　　要：目的探讨银杏叶提取物(GBE)对慢性阻塞性肺疾病(COPD)大鼠血清和肺泡灌洗液(BALF)中白细胞介素1(IL-1)、白细胞介素8(IL-8)含量的影响。方法将90只清洁级Wistar大鼠称重后,随机分为A、B、C、D、E、F组,每组15只。B、C、D、E、F 5组采用烟雾吸入等复合因素复制COPD大鼠模型;C、D组分别在1~14 d和29~42 d用GBE干预;E、F组分别在1~14 d和29~42 d用红霉素干预,实验结束取各组大鼠肺动脉血和BALF,检测各组血清和BALF中IL-1、IL-8的含量。结果 A、C、D、E、F组与B组相比,血清中IL-1、IL-8含量及BALF中IL-1含量低于B组(P<0.05)。在降低血清IL-1水平方面,早期GBE干预优于晚期;在降低大鼠血清和BALF中IL-1、IL-8水平方面,GBE与红霉素比较差异无统计学意义(P>0.05)。结论 GBE具有抑制COPD气道及全身炎症反应的作用。Objective To study the effect of Ginkgo biloba extract(GBE)on the content of IL-1 and IL-8 in the serum and bronchial alveolar lavage fluid(BALF)in rat model of chronic obstructive pulmonary disease(COPD).Methods In this study,90 Wistar rats were randomly divided into groups A,B,C,D,E and F with 15 rats in each group.The rat model of COPD was established by exposure to cigarette smoke and cold air stimulation composite factors in the groups B,C,D,E and F.The rats in the groups C and D were given intraperitoneal injection of GBE from day l to day l4 and day 29 to day 42 respectively.The rats in the groups E and F were given intraperitoneal injection of Erythromycin from day l to day l4 and day 29 to day 42 respectively.The serum and BALF of each group were collected after 43 days.Then the content of IL-1 and IL-8 in the serum and BALF was measured.Results Compared with the group B,the serum content of IL-1 and IL-8 and the content of IL-1 in the BALF were lower in the groups A,C,D,E and F(P<0.05).In reducing serum IL-1 level,early GBE intervention was better than late GBE intervention.In reducing serum and BALF IL-1 and IL-8 levels,GBE had no significant difference with Erythromycin(P>0.05).Conclusions GBE can inhibit the airway and systemic inflammation of COPD.

关 键 词：银杏叶提取物 慢性阻塞性肺疾病 白细胞介素-1 白细胞介素-8 

分 类 号：R541.5[医药卫生—心血管疾病]