肉牛源毛孢子菌Trichosporon loubieri的分离鉴定与药敏试验  

Isolation,identification and drug sensitive test of Trichosporon loubieri from beef cattle

作　　者：钟锐 姜尧章 马晓平[1] 左之才[1] 黄小丽[2] 邓俊良[1] 沈留红[1] 余树民[1] ZHONG Rui;JIANG Yaozhang;MA Xiaoping;ZUO Zhicai;HUANG Xiaoli;DENG Junliang;SHEN Liuhong;YU Shumin(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]2.四川农业大学动物科技学院,四川成都611130

出　　处：《浙江农业学报》2018年第1期26-35,共10页Acta Agriculturae Zhejiangensis

基　　金：四川农业大学科研兴趣计划项目(04051750);现代农业产业技术体系四川省肉牛创新团队

摘　　要：为探究肉牛真菌性皮肤病的病因并有效防治该病。取发生皮癣病肉牛患部的皮屑、被毛进行真菌学检查和分离。采用沙保弱氏培养法对皮屑和毛发样品进行分离纯化。对分离株进行形态学、分子生物学鉴定和小鼠致病性、药敏试验。最终从样本中分离到一株真菌(编号zrfg01)。对分离株进行PCR扩增,获得长度为542 bp的转录基因间隔区(intergenic spacer,IGS)序列片段,序列提交Gen Bank,获得序列号为MF401393.1。序列在Blast中比对结果显示,zrfg01与编号KT936593.1的Trichosporon loubieri的相似性为100%。根据形态学和分子生物学方法将其鉴定为T.loubieri。动物实验表明该菌对小鼠有选择性致病作用。药敏试验表明,该菌在25℃环境条件下,对特比萘芬和氟胞嘧啶耐药,对克霉唑、两性霉素B、氟康唑、伏立康唑较为敏感。该研究可为诊断和治疗T.loubieri引起的肉牛皮肤癣菌病提供重要参考。In order to have a deeper understanding of the causes of skin disease that beef cattle suffered and find the effective treatment,we collected the dandruff and hair of beef cattle skin with dermatophytosis then carried our an inspection and a separation of mycology.The dandruff and hair of beef cattle skin with dermatophytosis were collected and used for fungi culture.The morphological characteristics and IGS sequences of the isolated strains were identified.The pathogenicity test was carried out in mice,and drug sensitive test was performed.Finally,one strain of fungi,zrfg01,was obtained.The length of the IGS sequence was 542 bp amplified by PCR,which was submitted to GenBank(ID,MF401393.1).NCBI blast result showed that zrfg01 had 100%similarity with Trichosporon loubieri.According to the morphological identification,IGS sequence analysis and phylogenetic tree,the isolated strain was identified as T.loubieri.The pathogenicity test revealed that the fungus had a selective pathogenesis to mice.The drug susceptibility test showed that under the environment of 25℃,T.loubieri was resistant to terbinafine and fluorouracil,but sensitive to clotrimazole,amphotericin B,fluconazole and voriconazole.This study provided an important reference for the diagnosis and treatment of dermomycosis caused by T.loubieri

关 键 词：TRICHOSPORON loubieri 形态学 ITS序列 IGS序列 致病性 药敏试验 

分 类 号：S858.23[农业科学—临床兽医学] R379[农业科学—兽医学]