视神经损伤后JNK3、APP蛋白的表达变化对视网膜神经节细胞存活的影响  

The Expression Changes of JNK3 and APP Protein in Apoptosis of Retinal Ganglion Cells after Optic Nerve Injury

作　　者：赵瑜 赵安琪 陈晨[2] 王琳琳 ZHAO Yu;ZHAO An-qi;CHEN Chen;WANG Lin-lin(The Center of Biomedical Engineering Research,Kunming Medical University;Clinical Class 5 of 2015,Kunming Medical University;Key Laboratory of Stem Cells and Regenerative Medicine,Institute of Molecular and Clinical Medicine,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学生物医学工程研究中心 [2]昆明医科大学 [3]昆明医科大学分子临床医学研究院暨云南省干细胞和再生医学重点实验室,云南昆明650500

出　　处：《昆明医科大学学报》2018年第2期25-29,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2015FB013);云南省教育厅科学研究基金资助项目(2014Z073)

摘　　要：目的探讨视神经损伤后小鼠视网膜神经节细胞(RGCs)中JNK3、APP蛋白的表达变化及其对RGCs存活的影响.方法取成年APP野生型小鼠,采用钳夹法建立视神经损伤模型,应用免疫组织化学、Western blot法检测造模后视网膜神经节细胞中JNK3、APP蛋白的定位和定量表达变化,比较损伤7 d后APP基因敲除组及其同窝野生型小鼠RGCs的存活数变化.结果视神经损伤后,视网膜神经节细胞中JNK3定位于细胞浆,p-JNK则从细胞浆进入到细胞核中表达,APP定位于细胞膜、p-APP在轴突及细胞浆中均表达.同时,Western结果表明上述4种蛋白表达量在损伤1 d组比假手术组均升高(P<0.01).与APP野生型小鼠视网膜神经节细胞存活数(127.7±7.0)相比,APP基因敲除组小鼠视网膜神经节细胞存活数(147±7.0)明显增多(P<0.05).结论视神经损伤后,JNK3、APP蛋白的表达增多在视网膜神经节细胞的存活中发挥了一定的作用.Objective To investigate the changes in the expression of JNK3 and APP protein in retinal ganglion cells(RGCs)after optic nerve injury and its effect on the survival of RGCs.Methods Optic nerve crush models were established using adult APP wild type mice.Immunohistochemistry and western blot for detecting the expression of JNK3 and APP protein in RGCs were used post-injury.The number of RGCs in APP KO mice group was counted to compare with APP WT mice group.Results After optic nerve injury,JNK3 expressed in cytoplasm of RGCs;p-JNK transformed from cytoplasm to nuclei of RGCs;APP expressed in cytomembrane;p-APP expressed in cytoplasm and axons of RGCs.Western blot results showed that the protein expressions of JNK3,p-JNK,APP and p-APP were increased in the injured group(<0.01).The survival number of RGCs(147±7.0)in APP KO mice group increased significantly than APP WT mice group(127.7±7.0)(<<0.05).Conclusion The upregulation of JNK3 and APP protein expression maybe play an important role in RGCs survival proceeds after optic nerve injury.

关 键 词：JNK3 淀粉样前体蛋白 视网膜神经节细胞 

分 类 号：R318[医药卫生—生物医学工程]