西双版纳重要傣药大叶千斤拔总黄酮富集工艺研究  

Enrichment Method of Total Flavonoids of Important Dai Medicine Moghania macrophylla which Collected from Xishuangbanna

作　　者：牛迎凤 张丽霞[2] 李晓花[2] 柳觐[1] NIU Yingfeng;ZHANG Lixia;LI Xiaohua;LIU Jin(Yunnan Institute of Tropical Crops,Jinghong 666100,China;Yunnan Branch of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Jinghong 666100,China)

机构地区：[1]云南省热带作物科学研究所,云南景洪666100 [2]中国医学科学院药用植物研究所云南分所,云南景洪666100

出　　处：《热带农业科技》2018年第1期28-33,42,共7页Tropical Agricultural Science & Technology

基　　金：景洪市科技三项费专项([2013]48号);云南省热带作物科技创新体系建设项目(RF2017/RF2018)

摘　　要：以大叶千斤拔总黄酮含量为指标,考察大孔树脂、聚酰胺树脂、硅胶柱层析及溶剂萃取法对大叶千斤拔中总黄酮的富集效果。结果表明,乙酸乙酯萃取法和硅胶柱层析法联用能有效富集大叶千斤拔中的总黄酮。最佳纯化工艺为:大叶千斤拔提取液浓缩至无醇味,用2倍体积的乙酸乙酯萃取5次,所得萃取液浓缩蒸干后,用甲醇溶解至0.1 g/m L,拌样,上200-300目硅胶柱,上样量为1∶50,径高比为1∶15,用氯仿-甲醇(18∶1,v/v)的洗脱液洗脱,根据薄层结果,合并黄酮部分,浓缩蒸干后即得大叶千斤拔的黄酮提取物,纯度为54%。With the total flavonoids content ofMoghania macrophylla as indexes,the enrichment effect of macroporous resin,polyamide resin,silica gel column chromatography and solvent extraction method were compared.The combination usage of ethyl acetate extraction and silica gel column chromatography could enrich the total flavonoids of Moghania macrophylla effectively.The optimal purification process are as follow:The extracting solution of Moghania macrophylla was concentrated to no alcohol flavor,extracted five times with 2 times the volume of ethyl acetate;the extracted solution was dehydrated,dissolved in methanol to 0.1g/mL,the sample was mixed and loading to silica gel column of 200-300 mesh,the sampling amount was 1:50 and the diameter to height ratio of the column was 1:15,the eluent component was chloroform and methanol with the ration 18:1,the flavonoids was merged according to the results of the thin-layer chromatography and then dehydrated,the flavonoids concentration of Moghania macrophylla was 54%.

关 键 词：大叶千斤拔 总黄酮 富集工艺 

分 类 号：R282.71[医药卫生—中药学]