小RNA深度测序鉴定昭通市烟草脉斑病病毒  

Identification of the viruses from flue-cured tobacco in Zhaotong City by small RNA deep sequencing

作　　者：陶永萍 尹跃艳[2] 陈旭[1] 卢训[2] 李婷婷[2] 赵声春 游堂贵 丁铭[2] TAO Yongping;YIN Yueyan;CHEN Xu;LU Xun;LI Tingting;ZHAO Shengchun;YOU Tanggui;DING Ming(Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Laboratory of Agricultural Biotechnology,Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture,Kunming　650223,China;Yunnan Tobacco Company Zhaotong Branch,Zhaotong　657000,China)

机构地区：[1]云南省烟草公司昭通市公司,昭通657000 [2]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业部西南农业基因资源与种质创制重点实验室,昆明650223

出　　处：《植物保护》2018年第2期69-74,共6页Plant Protection

基　　金：中国烟草总公司云南省公司科技计划项目(2015YN21)

摘　　要：烟草脉斑病在云南昭通市的烟草上发生危害严重,为明确昭通市烟草脉斑病的病毒种类,本研究采集昭通市的4个烟草种植区症状表现为疑似烟草脉斑病的烟草样品,采用小RNA深度测序技术对不同来源的混合样品进行小RNA测序分析。结果显示混合样品中的病毒分别属于烟草花叶病毒属Tobamovirus、马铃薯Y病毒属Potyvirus和马铃薯卷叶病毒属Polerovirus。根据不同病毒属设计通用引物,分别对不同地区的烟草样品进行RT-PCR验证,结果表明,烟草样品中病毒种类有烟草花叶病毒、烟草脉带花叶病毒、烟草脉扭病毒和马铃薯Y病毒的PVYN、PVYN-Wi和PVYNTN株系。Flue-cured tobacco vein spot disease causes severe damage to tobacco production in Zhaotong City,Yunnan Province of China.In this study,siRNA deep sequencing was used to analyze the viruses in tobacco samples with necrotic and mosaic symptoms on tobacco leaves.The samples were collected from four different areas in Zhaotong City and mixed into one sample for siRNA sequencing analysis.The results showed that the identified viruses belonged to three genera,including Tobamovirus,Potyvirus and Polerovirus.RT-PCR and sequencing were used to identify the species of the viruses.Four viruses were detected in the samples,including TMV,TVBMV,TVDV and three strains of PVY(PVY N,PVY N-Wi,PVY NTN).

关 键 词：烟草脉斑病 烟草病毒 小RNA深度测序 

分 类 号：S432.41[农业科学—植物病理学]