花生茎线虫Ditylenchus arachis在真菌上的培养条件研究  

Cultural conditions of Ditylenchus arachis on fungi

作　　者：章淑玲[1] 林谷园[2] 肖顺[3] ZHANG Shuling;LIN Guyuan;XIAO Shun(Department of Horticulture and Garden,Fujian Vocational College of Agriculture,Fuzhou　350119,China;Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou　350001,China;College of Plant Protection,Fujian Agricultural and Forestry University,Fuzhou　350002,China)

机构地区：[1]福建农业职业技术学院园艺园林系,福州350119 [2]福建出入境检验检疫局,福州350001 [3]福建农林大学植物保护学院,福州350002

出　　处：《植物保护》2018年第2期100-103,121,共5页Plant Protection

基　　金：国家自然科学基金青年科学基金(31201495);福建省教育厅中青年项目(JA15759)

摘　　要：花生茎线虫Ditylenchus arachis是危害花生的重要病原线虫。本研究通过测试不同真菌和培养温度对花生茎线虫繁殖力的影响,以探讨该线虫在室内的培养条件。研究结果表明:花生茎线虫在长柄链格孢、茄镰孢、尖镰孢和灰葡萄孢上均能完成生活史并进行繁殖,但不同真菌上培养线虫的效果不同。其中长柄链格孢的培养效果最好,培养30d后的总虫量和繁殖系数分别为37 292条和1 243倍;灰葡萄孢的培养效果最差,30d后的总虫量和繁殖系数仅为1 514条和50倍。25~30℃是花生茎线虫的适宜培养温度,30℃时为最佳温度;当温度低于20℃或高于35℃时线虫繁殖效果差。此方法繁殖的线虫可用于花生茎线虫生物学、遗传学等方面的研究。Ditylenchus arachis is one of the important damaging nematodes of peanut in China.In order to study the cultural conditions on fungi in the laboratory,the effects of different fungi and different cultural temperatures on the reproductive capability of D.arachis were evaluated.The results showed that D.arachis could complete its life cycle and reproduce on Alternaria longipes,Fusarium solani,F.oxysporum and Botrytis cinerea,but the effects of different fungi varied.The reproductive capability of D.arachis was the highest on A.longipes,with a total number of nematodes and reproduction rate of 37 292 and 1 243,respectively.The total number of nematodes and reproduction rate were the lowest on B.cinerea(only 1 514 and 50,respectively).The optimum temperatures for D.arachis on fungi were from 25℃to 30℃,and 30℃was the best cultural temperature.D.arachis could not reproduce well at temperatures lower than 20℃or greater than 35℃.D.arachis cultured by this method can be used for biological and genetic studies on this nematode.

关 键 词：花生茎线虫 真菌 温度 繁殖力 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]