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作 者:赫卫[1] 张慧[1] 王莹 HE Wei;ZHANG Hui;WANG Ying(Institute of Horticulture,Heilongjiang Academy of Agricultural Sciences,Harbin 150069,China)
机构地区:[1]黑龙江省农业科学院园艺分院,哈尔滨150069
出 处:《植物保护》2018年第2期145-148,176,共5页Plant Protection
基 金:黑龙江省青年科学基金(QC2013C023);黑龙江省农业科学院引进博士人员科研启动金项目(201507-48);国家重点研发计划(2017YFD0101903);国家特色蔬菜产业技术体系(CARS-24-G-04)
摘 要:研究发现叶、茎、茎基部和果实接种4种方法均能用于鉴定辣椒对辣椒疫霉的抗性。叶与茎接种法操作简单,接种部位发病快,可同时检测大量品种,但要求空气湿度大;茎基部接种法模拟田间病害的发生过程,最能反映品种的真实抗性,但需要菌量大,工作量也大;果实接种法不仅操作简单、接种部位发病快、菌需求量小,而且可同时筛选大量品种,可以通过量化达到有效区分抗病和感病品种的目的,更具有完整保存原有植株的优势。By comparing different methods of leaf,stem,stem base and fruit inoculation,it was found that all the four methods could be used to identify the resistance of pepper to Phytophthora capsici.Leaf and stem inoculation methods are simple in operation and quick in infection,and could be used to detect a large number of varieties at the same time,but the high air humidity is required.The inoculation in stem base basically simulates the occurrence of field disease,which can reflect the real resistance of cultivars,but large amount of fungus and workload are required.Fruit inoculation method is not only simple in operation,quick in infection,small in inoculum size demand,but also can screen a large number of varieties at same time.The fruit method can be used for the effective distinction between resistant and susceptible varieties by quantifying,and has the advantage of preserving the original plant.
分 类 号:S436.418[农业科学—农业昆虫与害虫防治]
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