小鼠腹部异位心脏移植模型的建立与改进  

Establishment and improvement of abdominal heterotopic heart transplantation model in mice

作　　者：宋少华[1] 徐春扬 郭猛[1] 刘芳[1] 丁国善[1] 王全兴[2] 傅志仁[1] SONG Shaohua;XU Chunyang;GUO Meng;LIU Fang;DING Guoshan;WANG Quanxing;FU Zhiren(Organ Transplantation Center,Changzheng Hospital,Navy Medical University(Second Military Medical University),Shanghai 200003,China;Institute of Immunology,Navy Medical University(Second Military Medical University),Shanghai 200433,China)

机构地区：[1]海军军医大学(第二军医大学)长征医院器官移植中心,上海200003 [2]海军军医大学(第二军医大学)免疫学研究所,上海200433

出　　处：《第二军医大学学报》2018年第3期280-284,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(81270550;81470900;31670907);上海市卫生和计划生育委员会课题(201640274)~~

摘　　要：目的建立并改进小鼠腹部异位心脏移植模型,为器官移植免疫学研究提供支持。方法选取C57BL/6小鼠为同系移植供、受体和同种移植受体,BALB/c小鼠为同种移植供体。将供体心脏的升主动脉和肺动脉分别与受体的腹主动脉和下腔静脉行端侧吻合,建立小鼠腹部异位心脏移植模型,并对受体准备、供体心脏摘取和修整以及血管吻合技术加以改良,观察改进后的手术效果并分析其优势。结果预实验完成50对。正式实验同系间移植完成20对,成功17对,成功率为85.0%;同种间移植完成15对,成功13对,成功率为86.7%。模型总成功率为85.7%(30/35)。受体血管吻合前准备时间平均为(11.2±2.5)min,供体心脏摘取和修整时间平均为(13.6±3.3)min,供、受体血管吻合时间平均为(21.7±3.5)min。结论改进的小鼠腹部异位心脏移植模型具有简便、成功率高等优点,为进一步移植免疫学研究奠定了基础。Objective To establish and improve the abdominal heterotopic heart transplantation model in mice,so as to provide references for the research on organ transplantation immunology.Methods C57BL/6 mice were selected as donors and recipients for syngeneic cardiac transplantation and recipients for allogeneic cardiac transplantation;BALB/c mice were selected as donors for allogeneic cardiac transplantation.The ascending aorta and pulmonary artery of the heart of the donors were end-to-side anastomosed with the abdominal aorta and inferior vena cava of the recipients,respectively,to establish abdominal heterotopic heart transplantation models in mice.Furthermore,the techniques of preparing recipient heart before implantation,extracting and preparing donor heart,and vascular anastomosis were improved,and the surgical results and advantages were observed and analyzed.Results Preliminary experiments were performed on 50 pairs of mice.For formal experiments,syngeneic cardiac transplantation was performed on 20 pairs and the success rate was 85.0%(17/20);allogeneic cardiac transplantation was performed on 15 pairs and the success rate was 86.7%(13/15).The total success rate of abdominal heterotopic heart transplantation was 85.7%(30/35).The average time for preparing recipient before vascular anastomosis was(11.2±2.5)min,the average time for extracting and preparing donor heart was(13.6±3.3)min,and the average time for vascular anastomosis was(21.7±3.5)min.Conclusion The improved abdominal heterotopic heart transplantation model in mouse has the advantages of easy-to-perform and high success rate,which lays a foundation for the further study of transplantation immunology.

关 键 词：异位心脏移植 腹部 吻合 动物模型 

分 类 号：R332[医药卫生—人体生理学]