HPLC-TOF/MS快速鉴别相思藤中的化学成分  

Rapid identification of chemical constituents in Yine Abrus(Xiangsiteng) by HPLC-TOF/MS

作　　者：付振贺 黄玉凤[1] 黄超君 谢从景 吕磊[1] 杜红丽 赵亮[1] 焦杨[2] FU Zhenhe;HUANG Yufeng;HUANG Chaojun;XIE Congjing;LüLei;DU Hongli;ZHAO Liang;JIAO Yang(Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Navy Medical University(Second Military Medical University),Shanghai 200438,China;Department of Pharmacology,School of Pharmacy,Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]海军军医大学(第二军医大学)东方肝胆外科医院药材科,上海200438 [2]广西医科大学药学院药理教研室,南宁530021

出　　处：《第二军医大学学报》2018年第3期329-333,共5页Academic Journal of Second Military Medical University

摘　　要：目的采用高效液相色谱-飞行时间质谱(HPLC-TOF/MS)分析技术对中药材相思藤的化学成分进行快速鉴别分析。方法采用安捷伦"Formula-Database Generator"软件自建包括28个化合物的相思藤化学成分数据库,运用HPLC-TOF/MS技术采集相思藤提取物的总离子流色谱图,根据各化学成分的精确质荷比(m/z),通过自动比对的方法进行鉴别分析。色谱柱为安捷伦Zorbax Eclipse SB-C18(3.0 mm×100 mm,3.5μm)液相色谱柱,流动相为0.1%甲酸水溶液-乙腈,采用梯度洗脱,流速为0.4 m L/min,柱温25℃,紫外检测波长254 nm,进样量10μL;质谱采用电喷雾离子(ESI)源,正离子模式和负离子模式监测,质量数扫描范围为m/z 100~1 200。结果共鉴别出相思藤中15种化学成分,其中正离子模式下10种、负离子模式下11种,正、负离子模式下均有响应6种。结论建立了基于HPLC-TOF/MS的分析方法,可快速鉴别中药材相思藤中的化学成分,阐明了相思藤的化学物质基础,为相思藤的质量控制及药理机制研究奠定了基础。Objective To rapidly identify the chemical constituents in a traditional Chinese medicine Yine Abrus(Xiangsiteng)by high-performance liquid chromatography-time of flight mass spectrometry(HPLC-TOF/MS)analysis technique.Methods A formula database including 28 compounds of Xiangsiteng was developed by Agilent software“Formula-Database Generator”.The total ion chromatograms of the Xiangsiteng extracts were obtained by HPLC-TOF/MS,and the chemical components were identified by automatic matching method according to the exact mass-to-charge ratio of each chemical component.The separation was performed on a Agilent Zorbax Eclipse SB-C18 column(3.0 mm×100 mm,3.5μm).The mobile phase composing of acetonitrile and 0.1%formic acid aqueous solution was used for gradient eluting.The flowing rate was 0.4 mL/min;the temperature of column was 25℃;the UV detection wavelength was 254 nm;and the sample selection was 10μL.The electrospray ionization(ESI)source was applied for MS analysis under positive ion and negative ion modes,with the mass scan range being m/z 100-1 200.Results HPLC-TOF/MS identified a total of 15 chemical components from Xiangsiteng extracts,including 10 in positive ion mode and 11 in negative ion mode,and 6 both in both positive and negative ion modes.Conclusion A rapid and efficient method for identifying the chemical components of traditional Chinese medicine Xiangsiteng by HPLC-TOF/MS has been established,and the chemical components of Xiangsiteng has been clarified,which lays a foundation for the quality control and further pharmacological study of Xiangsiteng.

关 键 词：相思藤 高效液相色谱法 飞行时间质谱 化学成分 

分 类 号：R284.1[医药卫生—中药学]