二氢杨梅素保护ApoE^(-/-)动脉粥样硬化小鼠的作用及其机制  

Protective effect and mechanism of dihydromyricetin on atherosclerosis in ApoE^(-/-) mice

作　　者：王莹[1] WANG Ying(Tongliao Vocational College,Tongliao 028000,China)

机构地区：[1]通辽职业学院,内蒙古通辽028000

出　　处：《中成药》2018年第3期511-516,共6页Chinese Traditional Patent Medicine

摘　　要：目的探讨二氢杨梅素(dihydromyricetin,DHM)对高脂诱导的ApoE^(-/-)小鼠动脉粥样硬化的对抗作用及作用机制。方法取健康雄性ApoE^(-/-)小鼠30只,随机分为3组,即模型组、二氢杨梅素组[50 mg/(kg獉d)]和阿托伐他汀组[5 mg/(kg獉d)],每组10只;另取10只C57BL/6 J小鼠作为对照组。对照组与模型组小鼠予以0.5%羧甲基纤维素钠溶液灌胃,药物组小鼠灌以0.5%羧甲基纤维素钠溶液配制的药物混悬液,灌胃容积均为0.2 mL/10 g。同时所有小鼠均给予高脂饲料(0.3%胆固醇,20%脂肪)饲养。10周后,眼眶取血,分离血清,生化仪检测血脂4项水平,试剂盒检测血清氧化酶活性。取心脏油红O染色观察主动脉根部脂质沉积;免疫荧光检测斑块处巨噬细胞的堆积以及Caspase-3的表达;TUNEL试验检测主动脉瓣膜处巨噬细胞凋亡情况。结果连续灌胃10周后,与模型组相比,DHM组小鼠血清TC、TG和LDL-C水平显著下降,主动脉瓣膜处粥样硬化斑块显著减少;DHM组血清MDA水平明显降低,CAT、GSH-Px和SOD活性明显提高;主动脉根部细胞Caspase-3表达明显降低;巨噬细胞在动脉粥样硬化斑块处的堆积减少,TUNEL实验结果显示DHM组斑块处巨噬细胞的凋亡明显降低。结论二氢杨梅素能够改善高脂饲料诱导的ApoE^(-/-)动脉粥样硬化小鼠脂质堆积,其机制可能与抑制巨噬细胞凋亡有关。To explore the protective effect and mechanism of dihydromyricetin(DHM)on atherosclerosis(AS)in Apo-/-7 mice induced by high fat diet(HFD).METHODS Thirty healthy 6-week-old male Apo-/-7 mice were randomly divided into model group,DHM group[50 mg/(kg·d)]and Atorvastatin group [5 m g/(kg·d),i.g.],and another 10 male C57B L/6J mice were taken for control group.The mice in the control and model group were administered with 0.5%C MC-N a solution,while the other two groups were given DHM and atorvastatin suspension(0.2 m L/10 g).All mice went on with a 10-week H F D diet(0.3%cholesterol,20%fat),after which the orbit blood was procured for serum isolation and subsequent determination of levels of SOD,GSH-Px,CAT and MDA by the biochemical analyzer and test of lipid accumulation in the aotic root by oil red 0.The detection of macrophages accumulation and Caspase-3 expression level in the aortic root were achieved by immunofluorescence,and the macrophages apoptosis by TUNEL assay.RESULTS Significant post-treatment reduction in levels of T G,T C,and remarkable amelioration of LDL-C and plaque area in the aortic root were observed in DHM group when compared with model group.Meanwhile,DHM contributed to marked decrease of MDA level and improvement in activities of CAT,GSH-Px and SOD,the obvious macrophages accumulation prevention in atherosclerotic plaque.Additionally,its significant inhibition on macrophage apoptosis in aortic root was verified by the TUNEL assay.CONCLUSIONDHM reduces the lipid accumulation in HFD-induced ApoE-/-mice,mechanically a resultant of its inhibition on macrophage apoptosis.

关 键 词：二氢杨梅素 动脉粥样硬化 巨噬细胞 凋亡 APOE-/-小鼠 

分 类 号：R285.5[医药卫生—中药学]