黄芩-黄连药对防治D-半乳糖痴呆小鼠的作用机制  

Preventive and therapeutic mechanism of the herbal pair,Scutellariae Radix and Coptidis Rhizoma on dementia-like mice induced by D-galactose

作　　者：章常华[1] 刘彤彤 邓可众[1] 陈颖翀 林贵兵[1] 叶荷平[3] 涂秀英[1] 盛军庆[4] ZHANG Chang-hua;LIU Tong-tong;DENG Ke-zhong;CHEN Ying-chong;LIN Gui-bing;YE He-ping;TU Xiu-ying;SHENG Jun-qing(School of Pharmacy,Jiangxi University of TCM,Nanchang 330004,China;Ministry of Education Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Jiangxi University of TCM,Nanchang 330004,China;School of Life Sciences,Jiangxi University of TCM,Nanchang 330004,China;School of Life Sciences,Nanchang University,Nanchang 330031,China)

机构地区：[1]江西中医药大学药学院,江西南昌330004 [2]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004 [3]江西中医药大学生命科学学院,江西南昌330004 [4]南昌大学生命科学学院,江西南昌330031

出　　处：《中成药》2018年第3期524-529,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81460622,8774194,81260660);江西省自然科学基金(20l5lBAB205077,2017BAB20509095,20151BAB205079);江西省卫生和计划生育委员会科研计划项目(2016A024)

摘　　要：目的研究黄芩-黄连药对对D-半乳糖痴呆小鼠学习记忆能力的改善作用,并探讨其可能作用机制。方法皮下注射D-半乳糖100 mg/kg每天1次,连续8周诱导小鼠痴呆模型,与此同时分别灌胃黄芩-黄连药对5、10、20 g生药/kg,吡拉西坦0.75 g/kg,正常组给予等量蒸馏水。在给药后4、8周时用Morris水迷宫检测学习记忆能力,并于试验结束时测定血浆总超氧化物歧化酶(T-SOD)活性和丙二醛(MDA)水平,以及脑海马组织乙酰胆碱酯酶(Ach E)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果连续注射D-半乳糖4、8周后,黄芩-黄连药对能显著缩短D-半乳糖痴呆小鼠在定向航行和空间探索的潜伏期(P<0.05或P<0.01)。连续注射D-半乳糖8周,黄芩-黄连药对可非常显著升高血浆SOD活性和降低MDA水平(P<0.01),同时,可降低脑组织Ach E活性,并升高GSH-Px活性(P<0.01)。结论黄芩-黄连药对对老年性痴呆有一定的防治作用,其机理可能与其降低氧化应激水平和增加脑海马组织乙酰胆碱浓度有关。AIM To investigate the preventive effects of herbal pair,Scutellariae Radix and Coptidis Rhizoma(SC),on Alzheimer's disease(AD),and its mechanism of action.METHODS Dementia mice induced by 8-week s.i.d subcutaneous injection of D-galactose(100 mg/kg),were simultaneously given respective,intragastric administration of SC crude drug at doses of 5,10,20 g/kg,or piracetam support at 0.75 g/kg,and isometri-cal distilled water was applied to the mice of normal control group.The mice had their learning and memory abilities checked by Morris water maze at intervals of four weeks and eight weeks since the start of the trial,and their blood and brain tissue biochemical indices measured at the end of the test.RESULTS Significantly shortened latent period in place navigation test and the time of enter into the original platform in the space exploration test were observed in the mice treated with 4-week D-galactose and SC(P<0.05或P<0.01).The 8-week intervention demonstrated SC capacity in the significant promotion of T-SOD activity,decreased blood MDA levels(P<0.01)and the brain AchE levels,and increased brain GSH-Px activity(P<0.01).CONCLUSION SC increases the concentration of acetylcholine in brain tissue and protects the central nervous tissue under oxidative stress,highlighting its therapeutic effect on AD

关 键 词：黄芩-黄连药对 D-半乳糖 痴呆 改善 

分 类 号：R285.5[医药卫生—中药学]