姜黄素联合雷公藤红素体内外抗胃癌作用评价  

In vitro and in vivo anti-tumor effects of a combination therapy of curcumin and celastrol to gastric cancer

作　　者：苗金钰 佘君[1] 张玉磊 孙万森 MIAO Jin-yu;SHE Jun;ZHANG Yu-lei;SUN Wan-sen(School Hospital of XV an Jiaotong University,Xi'an 710049,China;Department of Traditional Chinese Medicine,The Second Hospital Affiliated to Xi'an Jiaotong University,Xi'an710049,China)

机构地区：[1]西安交通大学校医院,陕西西安710049 [2]西安交通大学第二附属医院中医科,陕西西安710004

出　　处：《中成药》2018年第3期549-553,共5页Chinese Traditional Patent Medicine

摘　　要：目的评价姜黄素联合雷公藤红素体内外抗胃癌作用及联用优势。方法 CCK-8法和流式细胞仪法检测姜黄素、雷公藤红素、姜黄素+雷公藤红素对人胃癌细胞BGC823细胞增殖的抑制及诱导凋亡作用;建立裸鼠BGC823细胞胃癌皮下移植模型,评价姜黄素、雷公藤红素、姜黄素+雷公藤红素给药后的抑瘤率、安全性及荷瘤裸鼠生存时间等指标。结果姜黄素、雷公藤红素、姜黄素+雷公藤红素均可抑制BGC823细胞增殖,IC_(50)分别为(57.47±5.22)、(2.11±0.07)、(0.22±0.01)μmol/L。姜黄素+雷公藤红素(10μmol/L+2μmol/L)可以诱导(73.07±3.82)%的BGC823细胞凋亡。姜黄素+雷公藤红素联合灌胃给药对BGC823细胞荷瘤裸鼠的抑瘤率为(64.87±4.16)%、中位生存时间为50.5 d,对体质量、肝指数和脾指数无显著毒副作用,血清中TNF-α和IL-6的水平显著升高。结论姜黄素联合雷公藤红素在体内和体外均有显著协同抗胃癌作用。AIM To evaluate anti-gastric cancer effects and benefits of the combination therapy of celastrol and curcumin in vitro and in vivo.METHODS Curcumin,celastrol and curcumin+celastrol applied to BGC823 gastric cancer cells had their impact on the cells growth and apoptosis in vitro checked by CCK-8 assay and flow cytometry of AnnexinV-FITC/PI;and their in vivo anti-tumor effect,systemic toxicity,and influence to the survival time evaluated by using BGC823-bearing gastric tumor mouse models induced by subcutaneous implantation.RESULTS IC50 of curcumin,celastrol and curcumin+celastrol on BGC823 cell were found to be at(57.47士5.22),(2.11±0.07)and(0?22±0.01)[xmol/L,respectively.Curcumin+celastrol achieved a most optimal BGC823 cell apoptosis rate of(73.07±3.82)%at concentrations of(10 mol/L+2 mol/L);and they left a positive impact on the BGC823 gastric tumor-bearing mice,given a(64.87±4.16)%inhibitory rate,and a 50.5-day median survival time.Additionally,the combination therapy demonstrated its less side effects in terms of the body weight,spleen and liver of the nude mice,and significantly improved serum concentrations of TNF-a and IL-6.CONCLUSION Combination of curcumin and celastrol shows significantly synergistic anti-gastric cancer effects in vitro and in vivo.

关 键 词：姜黄素 雷公藤红素 抗胃癌 联合治疗 

分 类 号：R285.5[医药卫生—中药学]