HPLC-ELSD法同时测定参麦止嗽糖浆中8种成分  

Simultaneous determination of eight constituents in Shenmai Zhisou Syrup by HPLC-ELSD

作　　者：谭雄斯[1] TAN Xiong-si(Zhaoqing Medical College,Zhaoqing 526020,China)

机构地区：[1]肇庆医学高等专科学校,广东肇庆526020

出　　处：《中成药》2018年第3期609-612,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC-ELSD法同时测定参麦止嗽糖浆(枇杷叶、麦冬、北沙参等)中蔷薇酸、委陵菜酸、马斯里酸、科罗索酸、麦冬皂苷D、麦冬皂苷D'、麦冬甲基黄烷酮A、麦冬甲基黄烷酮B的含有量。方法该药物甲醇提取液的分析采用Alltima C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相(乙腈-甲醇)-0.5%醋酸铵,梯度洗脱;体积流量0.7 mL/min;柱温35℃。结果 8种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.85%~99.43%,RSD 0.57%~1.33%。结论该方法简便、快速、准确,可用于参麦止嗽糖浆的质量控制。AIM To establish an HPLC-ELSD method for the simultaneous content determination of euscaphic acid,tormentic acid,crategolic acid,corosolic acid,ophiopogonin D,ophiopogonin D\methylophiopogonanone A and methylophiopogonanone B in Shenmai Zhisou Syrup(Eriobotryae Folium,Ophiopogords Radix,Glehniae Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35°C Alltima C1 8 column(4.6 mm X 250 mm,5(xm),with the mobile phase comprising of(acetonitrile-methanol)-0.5%ammonium acetate flowing at 0.7 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges(r>0.999 0),whose average recoveries were 96.85%-99.43%with the RSDs of 0.57%-1.33%.CONCLUSION This simple and accurate method can be used for the rapid quality control of Shenmai Zhisou Syrup.

关 键 词：参麦止嗽糖浆 化学成分 HPLC-ELSD 

分 类 号：R927.2[医药卫生—药学]