HPLC法同时测定17种石杉亚科植物石杉碱甲、乙  

Simultaneous determination of huperzine A and huperzine B in Seventeen species from Huperzioideae subfamily by HPLC

作　　者：赵惠[1] 覃兰芳 黄云峰 ZHAO Hui;QIN Lan-fang;HUANG Yun-feng(Guangxi University of Traditional Chinese Medicine,Nanning 530200,China;Guangxi Institute of Chinese Medicine&Pharmaceutical Science,Nanning 530022,China;Guangxi Key Laboratory of Traditional Chinese Medicine Quality Stardands,Nanning 530022,China)

机构地区：[1]广西中医药大学,广西南宁530200 [2]广西壮族自治区中医药研究院,广西南宁530022 [3]广西中药质量标准研究重点实验室,广西南宁530022

出　　处：《中成药》2018年第3期637-642,共6页Chinese Traditional Patent Medicine

基　　金：广西科学研究与技术开发计划项目(14123006-37);南宁市青秀区科学研究与技术开发计划项目(2013S13)

摘　　要：目的建立HPLC法同时测定17种(含2待发表新种)石杉亚科植物中石杉碱甲(huperzine A)和石杉碱乙(huperzine B)的含有量。方法石杉碱甲、乙酒石酸提取液的分析采用Wondasil C_(18)色谱柱(4.6 mm×250 mm,5μm);以甲醇-醋酸铵溶液为流动相,等度洗脱;体积流量1.0 mL/min;检测波长310 nm;柱温室温。外标法定量。结果石杉碱甲、乙分别在0.002 0~0.30μg、0.001 8~0.27μg范围内线性关系良好(r=0.999 9),平均加样回收率、RSD分别为103.86%(1.85%)、101.3%(1.30%)。17种石杉亚科植物中都含有石杉碱甲、乙,各物种之间含有量有显著性差异;喜马拉雅马尾杉中石杉碱甲含有量最高,达0.22%,柳杉叶马尾杉、有柄马尾杉和马尾杉含量也较高;马尾杉中石杉碱乙含有量最高。结论该方法准确稳定,重复性好,可用于石杉亚科植物中石杉碱甲和石杉碱乙的质量控制。AIM To establish an HPLC method for the simultaneous content determination of huperzine A and huperzine B from seventeen species Huperzioideae subfamily(including 2 ineditus new species).METHODS The analysises of tartaricacid extracts of huperzine A and huperzine B were performed on a room temperature column(4.6 mm X 250 mm,5(xm),with the mobile phase comprising of methanol-spiritus mindereri flowing at 1.0 mL/min in a isocratic elution manner,and the detection wavelength was set at 310 nm.The contents were determined by external standards.RESULTS Huperzine A and huperzine B showed good linear relationships within 0.002 0-0.30(xg,0.001 8-0.27(xg(r=0.999 9),whose average recoveries were 103.86%,101.3%with the RSDs of 1.85%,1.30%,respectively.Huperzine A and huperzine B were found in seventeen species,and there were significant differences in contents from species to species.The content of huperzine A in Phlegmariurus hamiltonii was the highest,which reached up to 0.22%,with higher levels of it in Phlegmariurus cryptomerianus,Phlegmariurus petiolatus,and Phlegmariurus phlegmaria;The content of huperzine B was the highest in P.phleg-maria.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of huperzine A and B from Huperzioideae subfamily.

关 键 词：石杉亚科 石杉碱甲 石杉碱乙 HPLC 

分 类 号：R284.1[医药卫生—中药学]