磺胺嘧啶锌(Ⅱ)配合物与牛血清白蛋白的相互作用研究  

Studay of interaction between Zn(SD)_2·2NH_3 and bovine serum albumin

作　　者：张恩风 张新波 黄富平[2] 刘宝 边贺东 雷福厚[1] ZHANG En-feng;ZHANG Xin-bo;HUANG Fu-ping;LIU Bao;BIAN He-dong;LEI Fu-hou(School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,College of Chemistry and Chemical Engineering,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Nanning 530006,China;State Key Laboratory for Chemistry and Moleculor Engineering of Medical Resources,Guangxi Normal University,Guilin 541004,China)

机构地区：[1]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西南宁530006 [2]广西师范大学,省部共建药用资源化学与药物分子工程国家重点实验室,广西桂林541004

出　　处：《化学研究与应用》2018年第3期319-326,共8页Chemical Research and Application

基　　金：国家自然科学基金项目(1061002,1361003,21101035)资助;教育部留学回国人员科研启动基金项目、广西自然科学基金项目(2016GXNSFDA380005,2011GXNSFC018009,2012GXNSFAA053035)资助;广西新世纪“十百千”人才计划项目资助

摘　　要：以Zn(Ⅱ)作为中心离子,磺胺嘧啶(HSD)作为配体合成了配合物Zn(SD)_2·2NH_3;利用荧光、红外差谱、圆二色谱等分析手段研究了配合物与牛血清白蛋白(BSA)的相互作用。结果表明,配合物可导致BSA内源性荧光猝灭,主要为静态猝灭。计算了在不同温度下相互作用的热力学参数和结合常数,推断其二者之间作用力为氢键和范德华力。光谱分析表明,配合物的加入使BSA的构象发生变化,α-螺旋结构含量降低。Zn(SD)2·2NH3 was synthesized using Zn(II)as the central ion and sulfadiazine(HSD)as ligand.The interaction between the complex and bovine serum albumin(BSA)was studied by the fluorescence spectra,circular dichroism and IR spectroscopy.Fluorescence spectroscopy results showed that the complex may lead to fluorescence quenching of BSA,which was mainly due to static quenching.The thermodynamic parameters and binding constants at different temperatures were calculated,indicating that the forces between them were hydrogen bonds and van der Waals forces.Analysis of IR spectroscopy and circular dichroism spectroscopy confirmed that the addition of complexes changed the conformation of BSA and reduced the content ofα-helix.

关 键 词：配合物 牛血清白蛋白 荧光光谱 圆二色光谱 红外光谱 

分 类 号：O614.24[理学—无机化学]