HBV感染肝癌患者外周血Treg细胞和IDO表达的相关性  

Expression and significance of treg cells and IDO in blood of the HBV-induced hepatocellar cancinoma

作　　者：杨峻[1,2] 王前[1] 石青峰[2] 苗海霞[3] YANG Jun;WANG Qian;SHI Qingfeng;MIAO Haixia(Southern Medical University,Guangzhou 510515,China;Department of Clinical Laboratory,Affiliated Hospital of Guilin Medical University,Guilin,Guangxi 541001,China;Guilin Entry Exit Inspection and Quarantine Bureau,Guilin,Guangxi 541000,China)

机构地区：[1]南方医科大学,广州510515 [2]桂林医学院附属医院检验科,广西桂林541001 [3]桂林出入境检验检疫局保健中心,广西桂林541000

出　　处：《国际检验医学杂志》2018年第3期257-259,263,共4页International Journal of Laboratory Medicine

基　　金：广西教育厅资助课题(0210150012);广西卫计委自筹经费课题(Z2012416)

摘　　要：目的探讨HBV感染肝癌患者外周血中Treg细胞和吲哚胺2,3-二氧酶(IDO)表达的相关性。方法对51例HBV-DNA阳性原发性肝癌(HCC)患者、44例慢性乙型肝炎(CHB)患者,29例肝硬化患者和28例健康志愿者(对照组)应用逆转录聚合酶链反应(RT-PCR)、流式细胞术检测各组外周血中Treg细胞和IDO的表达水平。结果 IDO mRNA表达从高到低依次为HCC组、CHB组、肝硬化组、对照组。对照组与肝硬化组IDO mRNA表达明显低于其他2组(P<0.01),其余各组之间比较,差异也有统计学意义(P<0.05)。Treg细胞计数从高到低依次为HCC组、肝硬化组、CHB组和对照组;HCC组明显高于对照组,差异有统计学意义(P<0.05),除CHB组外,各组Treg细胞与对照组比较,差异均有统计学意义(均P<0.05)。IDO mRNA、Treg表达相关性分析:r=0.912,P=0.000,表明患者外周血中IDO的表达与Treg细胞间呈正相关。结论HCC组中Treg细胞与IDO表达水平均高于对照组,肝癌患者外周血中IDO表达与Treg细胞间呈正相关,即IDO高表达伴随着Tregs比例增高。IDO和Treg细胞之间可能存在正反馈调节环路,二者共同诱导肝癌的免疫耐受,与肝癌的发生发展相关。Objective To investigate the clinical significance of the level of regulatory T(Treg)cells and indoleamine 2,3-dioxygenase(IDO)in HBV-induced hepatocellar cancinoma.Methods 51 cases of HBV-DNA positive primary liver cancer(HCC),44 cases of chronic hepatitis B(CHB),29 cases of cirrhosis and 28 cases of healthy volunteers(control group)were used RT-PCR and flow cytometry to detect the expression of Treg cells and IDO in peripheral blood of each group.Results The expression of IDO mRNA from high to low were HCC group,CHB group,liver cirrhosis group and control group.The control group and the liver cirrhosis group were lower than the other 2 groups(P<0.01),and the difference between the other groups was statistically significant(P<0.05).Treg counts from high to low were HCC group,cirrhosis group,CHB group and control group.HCC group was significantly higher than that of control group(P<0.05),except for CHB group,Treg cells in each group were statistically significant compared with control group(P<0.05).The correlation analysis of expression of IDO mRNA and Treg:r=0.912,P=0.000,indicating that the expression of IDO in peripheral blood was positively correlated with the Treg cells.Conclusion IDO is over expressed in HCC with the high Treg cell level.Treg cells and IDO may associated with hepatocellular carcinoma immune escape mechanisms.

关 键 词：肝癌 TREG细胞 吲哚胺2 3二氧酶 

分 类 号：R512.62[医药卫生—内科学] R735.7[医药卫生—临床医学]