葛根素对脑缺血再灌注大鼠海马组织P-STAT3、P53表达的影响  

Effects of Puerarin on the Expression of P-STAT3 and P53 in Hippocampus Tissue of Cerebral Ischemia-Reperfusion Injury Model Rats

作　　者：陈媛[1] 吴海金[1] 黄晓松[2] 罗银利[2] 王艳[2] 谭李红[3] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省第二人民医院,湖南长沙410007 [3]湖南省儿童医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2018年第1期36-39,共4页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅研究生创新课题(CX2015B338);湖南省中医药管理局一般项目(201653)

摘　　要：目的探讨葛根素对大鼠脑缺血再灌注损伤后海马组织磷酸化的转录激活蛋白3(STAT3)、凋亡因子P53表达的影响及其保护机制。方法采用线栓法制备大鼠左侧大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,利用葛根素干预,分别于缺血2 h再灌注6 h、24 h、72 h 3个不同时间点采用Longa评分法进行神经功能缺损评分、TUNEL法检测凋亡细胞数,免疫组织化学法检测海马组织磷酸化的STAT3(P-STAT3)及细胞凋亡因子P53的表达。结果与假手术组比较,葛根素组与缺血再灌注组的神经功能缺损加重,凋亡细胞数增多,P-STAT3和P53表达增高,差异均具有统计学意义(P<0.05);与缺血再灌注组比较,葛根素组神经功能缺损评分明显下降,凋亡阳性细胞数均见明显减少,P-STAT3和P53表达显著降低,差异均具有统计学意义(P<0.05)。结论脑缺血再灌注损伤后STAT3被异常激活,其磷酸化水平显著增高;葛根素可能是通过抑制STAT3的磷酸化水平,降低P53的表达起到抗神经细胞凋亡作用,从而改善大鼠脑组织的缺血性损伤。Objective To observe the effect of puerarin on the expression of phosphorylated transcription activator protein three(STAT3)and apoptosis factor P53 in the hippocampus tissue of cerebral ischemia reperfusion injury rats and to investigate its mechanism.Methods The middle cerebral artery occlusion(MCAO)model of left cerebra in rats were prepared by using suture method.The rats were given puerarin to intervene at 6 h,24 h,72 h for reperfusion after ischemia for 2 h.The neurologic impairment score was evaluated by Longa scoring,apoptotic cells number was detected by TUNEL method,and the expression of STAT3(P-STAT3)and apoptosis factor P53 in hippocampal tissue was detected by immunohistochemistry method.Results Compared with the sham-operation group,neurological impairment in puerarin group and cerebral ischemia-reperfusion group was more serious,the apoptosis numbers of hippocampus nerve cells and the expression levels of P-STAT3 and P53 increased significantly,the differences were statistically significant(P<0.05).Compared with cerebral ischemia-reperfusion group,the neurological impairment score,the apoptosis number of hippocampus nerve cells and the expression levels of P-STAT3 and P53 decreased,the differences were statistically significant(P<0.05).Conclusion STAT3 was activated abnormally after cerebral ischemic reperfusion injury,showing a significant increase in phosphorylation level.Puerarin could improve brain tissue damage,reduce the expression level of P53 and P-STAT3,and play a protective role in anti-apoptosis.

关 键 词：葛根素 脑缺血再灌注损伤 转录激活蛋白3 P53 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R743.3[医药卫生—中医学]