蛋白核小球藻对4种重金属胁迫的响应研究  

Responses of Chlorella pyrenoidesa to stress of four heavy metals

作　　者：王树磊[1] 陈珊 王英才[2] 史媛 张晶[2] WANG Shulei;CHEN Shan;WANG Yingcai;SHI Yuan;ZHANG Jing(Construction Management Bureau of South to North Water Diversion Project,Beijing 100038,China;Yangtze Valley Water Monitoring Center,Wuhan 430010,China)

机构地区：[1]南水北调中线干线工程建设管理局,北京100038 [2]江水利委员会长江流域水环境监测中心,湖北武汉430010

出　　处：《人民长江》2018年第6期19-25,共7页Yangtze River

摘　　要：为了利用藻类对水体重金属进行监测预警,以蛋白核小球藻为指示生物,研究了重金属Cd^(2+)、Cr^(6+)、Mn^(2+)、Pb^(2+)对藻类的抑制作用。实验结果表明:重金属对蛋白核小球藻的抑制作用总体表现为随着重金属浓度的增加和胁迫时间的延长而增加,抑制率一般在0~48 h内迅速增加,随后增长速率有所减缓,至96 h抑制率达到最大。蛋白核小球藻的抑制率与重金属浓度呈现明显的剂量-效应关系;与吸光度、叶绿素相对含量的变化相比,F_v/F_m对重金属胁迫的响应变化最为稳定和敏感,因此更适合作为检测指标监测重金属。从EC_(50)值的比较看,Cd^(2+)对蛋白核小球藻的毒性最大,其次是Cr^(6+)和Pb^(2+),Mn^(2+)的毒性最小。To monitor and early warn heavy metals in water by algae,we chose Chlorella pyrenoidesa as bioindicator to study its response to stress of four heavy metals Cd 2+,Cr 6+,Mn 2+,Pb 2+.It shows that the inhibition on Chlorella pyrenoidesa increases with the increase of metal concentration and reaction time in general.The inhibition rate rises sharply in 0~48 h.Thereafter,it rises slowly to the peak at 96 h.The inhibition rate of Chlorella pyrenoidesa and the heavy metal concentration demonstrates a does-effect relationship.Compared with the variation of the relative amount of absorption and chlorophyll,the response of F v/F m to stress of heavy metals is more stable and sensitive.Thus,it is more suitable to be the detection index.The EC 50 shows,the toxicity of Cd 2+to Chlorella pyrenoidesa is the highest,followed by Cr 6+and Pb 2+,and Mn 2+is the lowest.

关 键 词：生物毒性 重金属 蛋白核小球藻 水环境监测 

分 类 号：X832[环境科学与工程—环境工程]