杞黄颗粒对实验性脉络膜新生血管VEGF蛋白及其mRNA表达的影响  

Effect of Qihuang granule on experimental choroidal neovascularization VEGF Protein and its m RNA expression

作　　者：全颖 梁凤鸣[1] 王莉[1] 王燕[2] 黎红梅[1] 关玉双 王莹莹 QUAN Ying;IANG Fengming;WANG Li;WANG Yan;LI Hongmei;GUAN Yushuang;WANG Yingying(First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510000,China)

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]广东省中医院,广东510000

出　　处：《天津中医药大学学报》2018年第1期53-57,共5页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然基金面上项目(81373694)

摘　　要：[目的]探讨杞黄颗粒对激光诱导脉络膜新生血管(CNV)大鼠血管内皮生长因子(VEGF)蛋白及其mRNA表达的影响。[方法]81只雄性棕色挪威(BN)大鼠,采用随机分组法,13只为空白对照组;68只造模,随机抽取3只验证CNV形成,剩余65只随机分为5组:杞黄颗粒A组(简称杞黄A组),杞黄颗粒B组(简称杞黄B组),雷珠单抗组,杞黄颗粒+雷珠单抗组(简称杞黄+雷珠组),模型组。除杞黄B组给药90 d,其余组给药60 d后检测VEGF蛋白含量和mRNA表达水平。[结果]造模第7天,CNV验证形成。用药60 d,杞黄+雷珠组与杞黄A组、模型组相比VEGF的蛋白含量也明显降低,均P<0.05,具有统计学差异。杞黄B组与杞黄A组相比,VEGF蛋白含量降低降低,P<0.05,具有统计学意义。用药60 d,空白对照组相比模型组,杞黄+雷珠组相比模型组VEGF mRNA均有明显降低,P<0.05,具有统计学差异;杞黄B组相比杞黄A组的VEGF mRNA降低明显,P<0.05,具有统计学意义。[结论]杞黄颗粒与雷珠单抗联合使用,对于VEGF的表达有明显的调节作用,能够减少CNV的形成,对湿性老年性黄斑变性(WAMD)有一定治疗意义。[Objective]To investigate the effect of Qihuang granule on expression of vascular endothelial growth factor protein and its mRNA in laser-induced choroidal neovascularization.[Methods]Eighty-one male brown Norway rats were randomly divided into two groups:13 for the blank control group;68 were irradiated with 532 laser light.Three rats among the irradiated rats were randomly selected to the form of cnv and others were randomly divided into 5 groups:Qihuang granule group A(Gavage 60 days),Qihuang granule group B(Gavage 90 days),lucentis group,Qihuang granule combine lucentis group and the model group,with different drugs and time of treatment respectively.Expect Qihuang granule group B rats gavaged for 90 days,Others with 60 days,all retinal pigment epithelium-choroid-sclera complex form were geted to detect the protein content and mRNA expression of VEGF.[Results]After modeled 7 days,suggestting CNV formated.after 60 days intervention,VEGF protein content change,Qihuang granule combine lucentis group compared with Qihuang granule group A,Qihuang granule group A combine lucentis group compared with the the model group,VEGF protein content was reduced,with statistical differences(P<0.05).Qihuang granule group B compared with Qihuang granule group A,VEGF protein content were decreased,with statistically significant after 60 days intervention(P<0.05),VEGF mRNA change:compared the blank group with the model group,Qihuang granule combine lucentis group compared with the model group,VEGF mRNA were decreased with statistical differences(P<0.05).Qihuang granule group B compared with Qihuang granule group A,VEGF mRNA were decreased with statistically significant(P<0.05).[Conclusion]The method of Qihuang granule combine lucentis,can regulate the expression of VEGF,reduce the formation of CNV,and has a certain therapeutic significance to wet age-related macular degeneration.

关 键 词：杞黄颗粒 血管内皮生长因子 脉络膜新生血管 湿性老年性黄斑变性 

分 类 号：R285.5[医药卫生—中药学]