室内装修挥发性污染物对雄性小鼠生殖功能和精子凋亡的影响  

Influence of indoor decoration volatile pollutants in reproductive function and sperm apoptosis of male mice

作　　者：朱博 满枋霖 刘鹏 佟海滨[1] 田丹[1] 孙新[1] ZHU Bo;MAN Fanglin;LIU Peng;TONG Haibin;TIAN Dan;SUN Xin(Life Science Research Center,Beihua University,Jilin 132013,China;Reproduction Medicine Center,Affiliated Hospital,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学生命科学研究中心,吉林吉林132013 [2]北华大学附属医院生殖医学中心,吉林吉林132011

出　　处：《吉林大学学报（医学版）》2018年第2期281-285,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省卫计委科研计划项目资助课题(2013Z069);吉林省科技厅科技计划项目资助课题(20150312013ZG)

摘　　要：目的:模拟室内装修主要挥发性物质的污染环境,探讨室内装修主要挥发性污染物甲醛、苯系物及氨对雄性小鼠生殖功能的影响。方法:选取雄性ICR小鼠20只,随机平均分为实验组和对照组,实验组小鼠进行甲醛(60mg·m-3)、二甲苯(50mg·m-3)和氨(40mg·m-3)经呼吸道混配静式染毒,同时对照组小鼠放入空气填充的静式染毒柜中。每天固定时间称量小鼠体质量并记录,连续染毒35d。染毒期间每天观察小鼠行为学变化。收集小鼠附睾尾部精子并计数,检测精子密度及活力;采用Diff-Quick染色法染色,计算精子畸形率;采用流式细胞术(FCM)检测小鼠附睾尾部精子凋亡率;采用Western blotting法检测小鼠睾丸组织中细胞凋亡相关因子Caspase-9和Cleaved Caspase-3蛋白表达水平。结果:染毒28d后,实验组小鼠体质量与对照组比较明显降低(P<0.01);染毒35d后,实验组小鼠精子浓度、活力及精子畸形率与对照组比较均明显下降(P<0.01);与对照组比较,实验组小鼠精子凋亡率明显升高(P<0.01)。实验组与对照组小鼠睾丸组织中Caspase-9和Cleaved Caspase-3蛋白表达水平比较差异无统计学意义(P>0.05)。结论:室内装修主要污染物能够明显影响雄鼠附睾中精子质量。Objective:To simulate the pollution environment of indoor decoration volatile pollutants,and to explore the influence of three main indoor decoration volatile pollutants formaldehyde,xylene and ammonia in the male mouse reproductive function.Methods:A total of 20 male ICR mice were randomly divided into experimental group(exposed to 60 mg·m-3 formaldehyde,50 mg·m-3 xylene and 40 mg·m-3 ammonia mixed in the static type canister)and control group(put into the air-filled static type canister)(n=10).Each mouse was weighed at set times every day,exposed for 35 d continuously.The behavior changes of the mice were also observed everyday.The epididymal cauda sperms were collected to detect the sperm density and vitality.The sperm malformation rate was detected by Diff-Quick dyeing method.The apoptotic rate of epididymal cauda sperms was detected by flow cytometry(FCM).Then Western blotting method was used to determine the expression levels of Caspase-9 and Cleaved Caspase-3 proteins in testis tissue of the mice.Results:Four weeks after the exposure to pollutants,the body weight of the mice in experimental group was significantly lower than that in control group(P<0.01).The concentration and vitality of sperm malformation rate of the mice in experimental group were significantly lower than those in control group(P<0.01)35 d after exposure.Compared with control group,the apoptotic rate of sperm of the mice in experimental group was significantly increased(P<0.01).However,the expression levels of Caspase-9 and Cleaved Caspase-3 in the testis tissue of the mice in experimental group and control group had no significant differences(P>0.05).Conclusion:The main pollutants in indoor decoration can significantly influence the quality of spermatozoa in the epididymis.

关 键 词：甲醛 二甲苯 氨 精子 细胞凋亡 男性不育 

分 类 号：R697.22[医药卫生—泌尿科学]