10-23型脱氧核酶对HBV S、C基因体外切割作用研究  

Cleavage effect of type 10-23 deoxyribozyme on HBV S and C gene in vitro

作　　者：江京娣 张晓洁 张爱霞[1,2] 位春芳 徐茹 葛建顺 钱中清[1,2] 吕静竹[3] JIANG Jing-di;ZHANG Xiao-jie;ZHANG Ai-x;WEI Chun-fang;XU Ru;GE Jian-shun;QIAN Zhongqing;LV Jing-zhu(Bengbu Medical College Department of Biochemistry and Molecular Biology;Bengbu Medical College Department of Immunology;Bengbu Medical College Key Laboratory of Infection and Immunity,Anhui Provincial Key Laboratory;School of Clinical Medicine,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院生物化学与分子生物学教研室 [2]蚌埠医学院免疫学教研室 [3]蚌埠医学院感染与免疫安徽省重点实验室 [4]蚌埠医学院临床医学院,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2017年第12期1573-1575,共3页Journal of Bengbu Medical College

基　　金：国家自然科学基金项目(81570011);安徽省重点研究和开发计划(对外科技合作项目)(1604b0602026);安徽省高等学校省级自然科学研究重点项目(KJ2014A164);国家级大学生创新创业训练计划项目(201510367011)

摘　　要：目的:探讨10-23脱氧核酶(10-23 DRz)对HBV S基因及C基因体外转录产物的特异性切割作用。方法:分别构建含有HBV S基因或C基因的重组质粒,以线性化的重组质粒为模板,体外转录获得相应HBV S基因RNA及C基因RNA。设计并合成针对HBV S基因的DRz-hbvs-1和针对C基因的DRz-hbvc-1,观察其对靶RNA的体外切割作用。结果:DRz-hbvs-1及DRz-hbvc-1能对各自相应的靶RNA进行有效的特异性切割;无DRz对照组及反义寡核苷酸对照组均未见特异性切割。结论:10-23 DRz能特异性切割HBV S基因及C基因体外转录产物。Objective:To explore the specific cleavage activities of type 10-23 deoxyribozyme 10-23 DRz enzymes on HBV S and C RNA in vitro.Methods:The recombinant plasmids containing HBV S and C gene were constructed,respectively.The HBV S or C gene RNA was synthesized in vitro using the linearized recombinant plasmid as template.The DRz-hbvs-1 and DRz-hbvc-1 targeting to cleave HBV S and C mRNA were designed and synthesized,respectively,and the cleavage activities of which on targeting RNA were observed in vitro.Results:The DRz-hbvs-1 and DRz-hbvc-1 could specifically and effectively cleave the targeting RNA,and the cleavage of which in nonDRz and ASODN control groups were not found.Conclusions:The 10-23 DRz can specifically cleave the HBV S and C gene RNA in vitro.

关 键 词：乙型肝炎病毒 脱氧核酶 基因治疗 

分 类 号：R373.21[医药卫生—病原生物学]