桃红四物汤对大鼠血管吻合模型吻合区Delta-like4表达及血管新生的影响  

Effect of Taohong Siwu Tang(桃红四物汤) on Delta-like4 expression and angiogenesis in anastomosed area in vascular anastomose rat models

作　　者：金成鹏 孙绍裘[1] 彭志飞[1] 陈希龙[1] 曾景奇[1] 曾祥晶[1] 冯海波[1] 李益亮[1] JIN Chengpeng;SUN Shaoqiu;PENG Zhifei;CHEN Xilong;ZENG Jingqi;ZENG Xiangjing;FENG Haibo;LI Yiliang(The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,Hunan,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《中医正骨》2017年第12期1-5,共5页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：国家自然科学基金面上项目(81373658);国家中医药管理局全国名老中医药专家传承工作室建设项目;湖南省教育厅科学研究项目(15C1058)

摘　　要：目的:观察桃红四物汤对大鼠血管吻合模型吻合区Delta-like4表达及血管新生的影响。方法:将40只3月龄SPF级SD大鼠随机分为空白对照组、假手术组、生理盐水组、桃红四物汤组,每组10只。桃红四物汤组、生理盐水组行尾部动脉血管离断后吻合术;假手术组仅在相应区域作切口,不进行血管离断吻合;空白对照组不做任何处理。造模术后桃红四物汤组以20 mL·kg^(-1)剂量的桃红四物汤灌胃,生理盐水组给予等量生理盐水灌胃,每日灌胃2次;假手术组和空白对照组不给予任何药物干预。分别在药物干预开始后2 d、5 d从各组随机抽取5只大鼠,采用颈椎脱臼法处死后,桃红四物汤组和生理盐水组切取尾部的吻合处血管,假手术组和空白对照组切取尾部相应部位动脉血管。将所取血管组织制成石蜡切片,分成2份,1份采用免疫组化法检测血管吻合区Delta-like4的蛋白表达(光镜下灰度值与Delta-like4的蛋白表达量呈反比);1份经HE染色后测量吻合区血管内膜面积。结果:(1)血管吻合区Delta-like4的蛋白表达。药物干预开始后2 d,4组大鼠血管吻合区Delta-like4蛋白表达的灰度值比较,差异无统计学意义(126.27±20.56,111.73±7.99,119.30±16.71,124.53±7.35,F=0.624,P=0.062)。药物干预开始后5 d,4组大鼠血管吻合区Delta-like4蛋白表达的灰度值比较,差异有统计学意义(85.60±5.58,99.07±3.84,103.13±11.61,111.60±4.49,F=7.041,P=0.012);进一步两两比较,桃红四物汤组血管吻合区Delta-like4蛋白表达的灰度值低于生理盐水组(LSD-t=-2.334,P=0.048)、假手术组(LSD-t=-3.035,P=0.016)、空白对照组(LSD-t=-4.450,P=0.002);生理盐水组血管吻合区Delta-like4蛋白表达的灰度值与假手术组、空白对照组比较,差异均无统计学意义(LSD-t=-0.703,P=0.502;LSD-t=-2.166,P=0.062);假手术组血管吻合区Delta-like4蛋白表达的灰度值与空白对照组比较,差异无统计学意义(LSD-t=-1.464,P=0.181)�Objective:To observe the effect of Taohong Siwu Tang(桃红四物汤,THSWT)on Delta-like4 expression and angiogenesis in anastomosed area in vascular anastomose rat models.Methods:Forty 3-month-old SPF-grade SD rats were randomly divided into blank control group,sham-operated group,normal saline(NS)group and THSWT group,10 rats in each group.The surgery of mutilation-anastomose were performed on the caudal artery of rats in THSWT group and NS group,and sham operation were performed on rats in sham-operated group,while the rats in blank control group were not given any surgical intervention.After the end of the modeling operation,the rats in THSWT group were intragastric administrated with THSWT in dosage of 20 ml/kg,and the rats in NS group were intragastric administrated with the same dose of normal saline,twice a day.The rats in sham-operated group and blank control group were not given any drug intervention.At 2 and 5 days after the beginning of drug intervention,5 rats were randomly selected from each group respectively and were executed by using cervical dislocation method.The caudal anastomotic blood vessels of rats were fetched in THSWT group and NS group,and the caudal arterial vessels of rats in corresponding sites were fetched in sham-operated group and blank control group.The acquired vascular tissues were made into paraffin sections and were divided into 2 parts,one of them was applied to measure the Delta-like 4 protein expression by using immunohistochemical method(the gray values were inversely proportional to the delta-like4 protein expression under light microscope),while the other portion was applied to measure the area of endangium in anastomosed area through HE staining.Results:At 2 days after the beginning of the drug intervention,there was no statistical difference in the gray values of Delta-like 4 protein expression in vascular anastomosed area between the 4 groups(126.27+/-20.56,111.73+/-7.99,119.30+/-16.71,124.53+/-7.35,F=0.624,P=0.062).At 5 days after the beginning of the drug intervent

关 键 词：桃红四物汤 血管新生 受体 Notch Delta-like4 大鼠 动物实验 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]