小麦抗白粉病基因pm30种质改良及鉴定  

Enhancement and Molecular Identification of Wheat Germplasm Resources with Resistance Gene Pm30 to Powdery Mildew

作　　者：兰素缺[1] 李光威[1] 孟雅宁[2] 李芳 张业伦[1] 李杏普[1] LAN Suque;LI Guangwei;MENG Yaning;LI Fang;ZHANG Yelun;LI Xingpu(Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Hebei Laboratory of Crop Genetics and Breeding,Hebei Wheat Innovation Team of Modern Agro-industry Technology Research System,Shijiazhuang 050035,China;Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

机构地区：[1]河北省农林科学院粮油作物研究所,河北省作物遗传育种实验室,河北省现代农业产业技术体系小麦创新团队,河北石家庄050035 [2]河北省农林科学院经济作物研究所,河北石家庄050051

出　　处：《华北农学报》2017年第A01期7-11,共5页Acta Agriculturae Boreali-Sinica

基　　金：小麦种质资源发掘与创新利用(2013BAD01B02-11);小麦抗白粉病种质资源创新(11220112D);小麦种质资源表型精准鉴定与优质、抗病等种质创新(2016YFD0100102-5);冬小麦种质资源与育种技术创新(16226320D)

摘　　要：白粉病是威胁我国乃至世界小麦生产的最主要病害之一。筛选和培育抗病品种是预防小麦感病减产的一条安全有效途径。为了将合成六倍体小麦中的白粉病抗病基因Pm30导入综合农艺性状好、产量高的优良小麦品种中,利用自育中抗白粉病的优良品系冀资356与合成小麦Synthetics(He-48)(T.dicoccon PI94625/Ae.squarrosa)杂交,经过连续6年的选育,选育出高抗白粉病且农艺性状优良的品系7份。利用与Pm30连锁的SSR标记Xgwm159对这7份品系进行检测,用中国春和Pm30做对照,结果表明,14YF650和14YF675 2个品系扩增出430,460,500 bp条带,说明这2份材料携带来自于野生二粒小麦的抗白粉病基因Pm30。这2个品系表现矮秆抗倒、大穗、综合农艺性状较好,可以作为抗白粉病的亲本直接应用于抗病育种。Wheat powdery mildew is one of the main diseases threaten wheat production in China even all over the world.Screening and development disease-resistant varieties is a safe and effective way for prevention and control of wheat powdery mildew.The purpose of this study is to introduce powdery mildew resistance gene Pm30 into the wheat varieties Jizi 356 with advanced comprehensive agronomic characters and high yield developed by authors.One crosses,Jizi 356/Synthetics He-48 were made.Twelve lines with field resistance to powdery mildew and excellent agronomic characters were developed after six years of offspring screening.Then,seven lines were used for identifying resistance gene Pm30 using Xgwm159 SSR marker.Chinese spring and Pm30 were used as a contrast.The results showed that two new lines 14YF650 and 14YF675 could amplify the 430,460,500 bp polymorphic fragments.It indicated the two lines carrying Pm30 powdery mildew resistance gene derived from wild emmer.Those lines with big spikes,resistant to lodging and excellent agronomic characters could be used directly as parents for wheat powdery mildew resistance breeding.

关 键 词：普通小麦 野生二粒小麦 抗病基因 白粉病 Pm30 分子标记 

分 类 号：Q78[生物学—分子生物学] S512.03[农业科学—作物学]