高粱丝黑穗病抗性基因SRAP标记的初步分析  

Preliminary Analysis on SRAP Markers for Head Smut Resistance in Sorghum

作　　者：段永红 张旭[2] 崔福柱[2] 司浩浩[2] 孙毅 张福耀[1] DUAN Yonghong;ZHANG Xu;CUI Fuzhu;SI Haohao;SUN Yi;ZHANG Fuyao(Key Laboratory of Sorghum Genetic and Germplasm Innovation of Shanxi Province,Jinzhong 030600,China;College of Agronomy,Shanxi Agricultural University,Taigu 030801,China;Biotechnology Research Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China;Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030031,China)

机构地区：[1]高粱遗传与种质创新山西省重点实验室,山西晋中030600 [2]山西农业大学农学院,山西太谷030801 [3]山西省农业科学院生物技术研究中心,山西太原030031 [4]农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031

出　　处：《华北农学报》2017年第A01期12-16,共5页Acta Agriculturae Boreali-Sinica

基　　金：高粱遗传与种质创新山西省重点实验室开放课题(2015K-03);山西省自然科学基金项目(201701D121105);山西省财政支农项目(2015TGSF-10);国家公益性行业(农业)科研专项(201503121-07)

摘　　要：为筛选与高粱抗丝黑穗病基因相连锁的SRAP分子标记,实现实验室内对高粱抗病性的选择鉴定,达到真正意义的分子标记辅助育种。选用高粱感病品种三尺三和抗病品种Tx622B为研究材料,观察了其形态学和农艺性状,筛选了具有多态性的SRAP标记。结果表明:高粱品种三尺三与Tx622B的田间性状有明显的差异,三尺三的株高、茎粗、秆高、地面茎节数、穗长、穗节数、穗粒重和千粒质量均高于Tx622B,穗一级分支数高于Tx622B,而小区穗数低于Tx622B。2个品种产量性状的差异达到极显著的水平。筛选出16对在抗感品种间有多态性的引物,可用于高粱抗丝黑穗病基因研究。将为高粱抗丝黑穗病优良品种的筛选、培育提供研究基础。For screening SRAP markers associated to head smut resistance in sorghum,and to select and identify resistant sorghum in the laboratory,achieving the real molecular marker-assisted breeding,a sensitive sorghum variety Sanchisan,and a resistant variety Tx622B were selected,as research materials.Their morphological and agronomical characters were observed,and the polymorphic SRAP markers were screened.The results showed that there were significant differences in the field characters between Sanchisan and Tx622B,the plant height,stem diameter,culm height,numbers of stem nodes above ground,panical length,numbers of panicle nodes,panicle kernel weight and 1 000 kernel weight of Sanchisan were higher than Tx622B,its primary branch number was higher than Tx622B,in plot was less than Tx622B;The difference of yield traits between the two varieties reached a very significant level.And 16 pairs of polymorphic primers were identified between the two varieties,which can be further used for identifying head smut resistance-associated genes in sorghum.It provided basis for selecting and breeding resistant sorghum varieties to head smut.

关 键 词：高粱 丝黑穗病 SRAP标记 抗性基因 

分 类 号：S332.2[农业科学—作物遗传育种] Q75[农业科学—农艺学]