四川地区猪场中耐甲氧西林金黄色葡萄球菌的SCCmec基因分型与耐药性分析  

Antimicrobial Susceptibility and SCCmec Genotyping of Methicillin Staphylococcus aureus Isolates from Swine in Sichuan

作　　者：许思遥 黄仆[1] 李萍[1] 杨凡[1] 姜子义[1] 吕雯婷[1] 徐志文[1,2] 朱玲[1,2] XU Siyao;HUANG Pu;LI Ping;YANG Fan;JIANG Ziyi;LV Wenting;XU Zhiwen;ZHU Ling(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Key Laboratory of Animal Disease and Human Health of Sichuan Province Science&Technology Department of Sichuan Province,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《华北农学报》2017年第A01期23-29,共7页Acta Agriculturae Boreali-Sinica

基　　金：四川省科技支撑计划项目(2014NZ0043);四川省"十三五"育种攻关项目(2016NYZ0052)

摘　　要：为研究四川猪场分离的耐甲氧西林金黄色葡萄球菌(MRSA)的耐药现状及其SCCmec基因型情况,讨论猪源MRSA对公共卫生带来的隐患。于2013年3-12月在四川地区12个规模化猪场采集的120份样品中分离金黄色葡萄球菌(SA),对PCR初步鉴定出SA,再鉴定mec A、fem A耐药基因,阳性菌株判定为MRSA。对鉴定出的猪源MRSA进行SCCmec分型与耐药性试验及分析。共分离出60株金黄色葡萄球菌,其中22株MRSA,检出率18.3%(22/120),饲养员手棉拭子、猪鼻捻子、猪场水样中均分离出MRSA。药敏试验结果表明,22株MRSA菌株的多重耐药性明显,耐药性主要表现为4、6耐,其中SCCmecⅣa型17株、SCCmecⅠ型5株。目前四川地区猪源MRSA不仅对猪场人员存在感染隐患,同时也对公共卫生安全存在一定的威胁。To study the drug resistance of SCCmec resistant Staphylococcus aureus and analyze pubic health in Sichuan,we collected 120 samples from Sichuan pig-farms to identify MRSA by PCR test from Sichuan.Then identified these 22 MRSA by drug-resistance testing and SCCmec typing.In these samples,60 strains of Staphylococcus aureus had been isolated from pig water and the hand of worker,including 22 MRSA,the detection rate was 18.3%(22/120).Susceptibility testing results showed that this 22 multi-drug resistant MRSA strains had significant multi-drug resistance,most were 4,6-resistant.Genotyping results showed that the predominant type of the Sichuan MRSA were mainly SCCmecⅣa type,including 17 strains of SCCmecⅣa type and 5 strains of SCCmecⅠtype.Last discuss the MRSA from swine have imperiled the health of worker and public health.

关 键 词：耐甲氧西林金黄色葡萄球菌 SCCmec分型 耐药性 公共卫生 

分 类 号：Q78[生物学—分子生物学] S442[农业科学—农业昆虫与害虫防治]