玫瑰黄链霉菌Men-myco-93-63发酵液防治黄瓜白粉病的作用机理研究  

The Study on Resist Mechanism Induced by Fermentation Broth of Stretomyces roseoflavus Men-myco-93-63 Against Cucumber Powdery Mildew

作　　者：郭敬华[1,2,3] 孟庆芳 石琳琪[3] 刘大群[1,2] GUO Jinghua;MENG Qingfang;SHI Linqi;LIU Daqun(College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China;Biological Control Center of Plant Diseases and Plant Pests of Hebei Province,Baoding 071001,China;Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

机构地区：[1]河北农业大学植物保护学院,河北保定071001 [2]河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [3]河北省农林科学院经济作物研究所,河北石家庄050051

出　　处：《华北农学报》2017年第A01期69-75,共7页Acta Agriculturae Boreali-Sinica

基　　金：河北省重点研发计划项目“有机生态型基质栽培关键技术集成创新及示范推广”;河北省现代农业产业技术体系(HBCT2013050203);石家庄市科学技术研究与发展计划项目(151490362A)

摘　　要：利用透射电镜技术与细胞学相结合的方法探索了Men-myco-93-63发酵液对黄瓜白粉病菌的作用位点,揭示了白粉菌以及寄主细胞在超微结构上发生的变化。利用扫描电镜技术研究了处理前后黄瓜白粉病菌孢子萌发率、菌丝的生长、产孢时间。结果表明:利用透射电镜观察超微结构表明,寄主细胞对病原菌的侵入产生了防卫反应结构,表现为寄主细胞壁加厚,染色加深,在寄主细胞壁下产生结构致密的乳突,在寄主细胞壁与质膜之间有黑色沉积物质。黄瓜白粉菌产生吸器的数目明显减少,吸器畸形,吸器壁增厚,细胞器泡囊化解体,最终吸器坏死,不能从寄主细胞中更好地吸取营养,达到防病的目的。利用扫描电镜观察表明,Men-myco-93-63发酵液使白粉菌的孢子萌发率降低31.51%,在一定程度上抑制了菌丝的生长,寄主体表的菌丝形态表现为菌丝扭曲变形,菌落稀疏变小,延迟其产孢时间约2 d。Men-myco-93-63发酵液能通过直接对菌体作用达到防治黄瓜白粉病的目的。To explore the control mechanism of fermentation broth of Stremptomyces roseoflavus Men-myco-93-63 on cucumber powdery by means of electronic microscopy as well as cytochemical technology,then,Ultrastructure changing of the host cell wall and Sphaerotheca fuliginea(Schlecht.)Poll.was studied.Scanning electron microscopy was employed to investigate the spore germination,growth of mycelium,sporulation time treated by fermentation broth of Men-myco-93-63.The results showed that by means of TEM,being treated with fermentation liquid of Men-myco-93-63 against cucumber powdery mildew,the host cells produced defense structures and material related to infection,it showed that the host cell wall was thickened and deeply stained,solidity of papilla structure produced under the cell wall,dark material deposited between the cell wall and plasmalemma.The number of haustellum of Sphaerotheca fuiginea was reduced obviously,haustoria became malformed and haustorial wall thickened,organelles disintegrated,haustoria necrotizcd finally,the haustoria can′t absorb favourably nutrition from the host.By means of scanning electron microscopy which employed hat the fermentation broth of Men-myco-93-63 reduced sphaerotheca fuiginea(Schlecht.)Poll germination 31.51%.The amount of hypha were restrained a certain extent after treatment by the fermentation broth of Men-myco-93-63.The hypha on the host surface had taken place a series of change,such as hypha distortion,clone sparsing and decreesinsing,and which delayed come into being sporule about two days.The control effect on powdery mildew was confirmed by observation with scanning electron microscopy.

关 键 词：黄瓜白粉病 Men-myco-93-63发酵液 抗病机理 超微结构 电镜 菌丝 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治]