扰动强度对太湖水华微囊藻群体生长和叶绿素荧光的影响  

Effect of Disturbance Intensity on the Growth and Chlorophyll Fluorescence of Microcystis flos-aquae Colony in Lake Taihu

作　　者：韩丽华 杨桂军[1] 刘玉 秦伯强[2] 钟春妮 杨宏伟[2] HAN Lihua;YANG Guijun;LIU Yu;QIN Boqiang;ZHONG Chunni;YANG Hongwei(Environment and Civil Engineering School,Jiangnan University,Wuxi 214122,China;State Key Laboratory of Lake and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences,Nanjing 210008,China)

机构地区：[1]江南大学环境与土木工程学院,江苏无锡214122 [2]中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室,江苏南京210008

出　　处：《环境科学研究》2018年第2期265-272,共8页Research of Environmental Sciences

基　　金：国家自然科学基金重点项目(No.41230744);国家水体污染控制与治理科技重大专项(No.2012ZX07503-002,2012ZX07101-013-03)

摘　　要：风浪扰动在湖泊和水库中频繁发生,是影响湖泊生态系统的重要因素之一.为了解太湖风浪扰动对水华微囊藻群体生长的影响,并探究其影响机理,利用室内摇床试验,设置不同的扰动强度(0、50、100、200、400 rmin)来模拟太湖风浪扰动,扰动时间为24 h,并测定不同扰动强度下水华微囊藻群体生长和叶绿素荧光参数.结果表明,所有试验组中,100 rmin组的ρ(Chla)、微囊藻数量、F_vF_m(潜在最大光合效率)、ETR_(max)(潜在最大光合速率)、I_k(半饱和光强)和α(光能利用效率)增加最快,试验结束时分别为扰动前的3.29、10.75、1.20、2.30、2.21和1.21倍;并且扰动结束后,100 rmin组中3~10细胞群体细胞数量占比由25.80%降至20.70%,显著低于对照组(P<0.05),而>10细胞群体细胞数量占比由0增至25.55%,显著高于对照组(P<0.05).方差分析表明,试验第7~11天,100 rmin组的ρ(Chla)、水华微囊藻数量、F_vF_m、ETR_(max)和α显著高于对照组(P<0.05);第1~5天,400rmin组的ρ(Chla)、ETR_(max)和I_k显著低于对照组(P<0.05).研究显示,适宜的扰动强度(100 rmin)促进水华微囊藻群体生长和光合活性,过高的扰动强度(400 rmin)则抑制水华微囊藻群体生长和光合活性.Disturbance induced by wind-wave occurs frequently in lakes and reservoirs,and it is one of the important factors affecting the lakes ecosystem.To understand the effect and mechanism of mixing induced by wind-wave on the growth of Microcystis flos-aquae colony in Lake Taihu,an experiment was conducted in lab,using different disturbance intensity(0,50,100,200 and 400 r min)by agitator to simulate the mixing induced by wind-wave in Lake Taihu.The disturbance time is 24 h.Growth and chlorophyll fluorescence parameters of M.flos-aquae colony were measured in this study.The study results showed that the increasing speeds ofρ(Chla),abundance,potential maximum photosynthetic efficiency(F v F m),the potential maximum photosynthetic rate(ETR max),half saturation light intensity(I k)and light use efficiency(α)of M.flos-aquae in the 100 r min groups were the highest among all treatment groups.In the end of the experiment,the value ofρ(Chla),abundance,F v F m,ETR max,I k andαof M.flos-aquae in the 100 r min groups were 3.29,10.75,1.20,2.30,2.21 and 1.21 times as more as that before mixing in the experiment.In the end of this experiment,the cell abundance of 3-10 cells colony of M.flos-aquae in the 100 r min groups decreased from 25.80%to 20.70%to total cell abundance,and which was significantly lower than that in the control groups(P<0.05).However,the cell abundance of>10 cells colony of M.flos-aquae in the 100 r min groups increased significantly from 0 to 25.55%to total cell abundance,which was significantly higher than that in the control groups(P<0.05).The variance analysis showed that theρ(Chla),abundance,F v F m,ETR max andαof M.flos-aquae in the 100 r min groups were significantly higher than those in the control groups(P<0.05)during the 7 th-11 th day in the experiment.During the 1 st-5 th day,theρ(Chla),ETR max and I k in the 400 r min treatment groups were significantly lower than those in the control groups(P<0.05)in the experiment.The results showed that the suitable disturbance intensity(100 r min)promote

关 键 词：水华微囊藻 群体 扰动强度 叶绿素荧光 太湖 

分 类 号：X52[环境科学与工程—环境工程]