邻苯二甲酸二辛酯亚慢性暴露对SD大鼠血细胞的影响  

Toxicity to blood cells of SD rats from sub-chronic exposure to DEHP

作　　者：任雪丹[1,2] 李诺[1,2] 吕中明[2] 王民生[2] REN Xuedan;LI Nuo;LV Zhongming;WANG Minsheng(School of Public Health,Southeast University,Nanjing 210009;Institute of Pharmacology and Function Evaluation,Jiangsu Center for Disease Control and Prevention,Nanjing 210009,Jiangsu,China)

机构地区：[1]东南大学公共卫生学院,江苏南京210009 [2]江苏省疾病预防控制中心,江苏南京210009

出　　处：《癌变．畸变．突变》2018年第2期140-143,149,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：东南大学环境医学工程教育部重点实验室开放课题(2015 EME002);江苏省普通高校研究生科研创新计划(SJZZ15_0026)

摘　　要：目的:探讨邻苯二甲酸二辛酯(DEHP)对大鼠血细胞的影响。方法:SD大鼠设对照组(饲喂基础饲料)、4个DEHP染毒组(分别为5、50、500和2 500 mg/kg),每组20只,雌雄各半,连续饲喂染毒90 d。染毒结束后,取大鼠全血测定各类血细胞的变化。结果:与对照组相比,白细胞参数中,2 500 mg/kg组雄性大鼠嗜酸性粒细胞显著减少(P<0.01),雌性大鼠白细胞总数显著降低(P<0.05);500和2 500 mg/kg组雌性大鼠淋巴细胞显著增加(P<0.05)。红细胞参数中,2 500 mg/kg组雄性大鼠红细胞总数、血红蛋白(HGB)和红细胞压积(HCT)显著下降(P<0.01);雌性大鼠500和2 500 mg/kg组血红蛋白(HGB)显著下降(P<0.05);500和2 500 mg/kg组的平均血红蛋白含量(MCH)显著下降(P<0.05或P<0.01);2 500 mg/kg组的平均血红蛋白浓度(MCHC)和直接测定的血红蛋白浓度(CHCM)显著下降(P<0.05或P<0.01)。未发现DEHP对雌雄大鼠血小板各指标产生影响。结论:一定剂量条件下,DEHP可对大鼠白细胞和红细胞相关指标产生影响。OBJECTIVE:To observe toxicological effects in blood cells in rats after treatment with different doses(0,5,50,500,2500 mg/kg)DEHP.METHODS:Rats were divided into control and four treatment groups.The treatment groups were exposed to four concentrations of DEHP(5,50,500 and 2500 mg/kg)for 90 days.At the end of the exposure,whole blood samples were taken to measure the changes of various types of blood cells.RESULTS:Compared with the control group,the leukocyte parameters for the 2500 mg/kg dose groups were:eosinophils decreased significantly(P<0.01),the total number of white rats significantly decreased(P<0.05).Lymphocytes in 500 and 2500 mg/kg groups were significantly increased(P<0.05).In the erythrocyte parameters,the total number of red blood cells,hemoglobin(HGB)and hematocrit(HCT)in the 2500 mg/kg group were significantly decreased(P<0.01)while the levels of hemoglobin(HGB)in 500 and 2500 mg(P<0.05 or P<0.05).Mean hemoglobin content(MCH)in 500 and 2500 mg/kg groups decreased significantly(P<0.05 or P<0.01).The measured hemoglobin concentration(CHCM)decreased significantly(P<0.05 or P<0.01).On the other hand,DEHP had little effect on platelet parameters.CONCLUSION:DEHP exposure affected blood cells of rats under certain dosages.

关 键 词：DEHP 大鼠 白细胞 红细胞 血小板 

分 类 号：R994.6[医药卫生—毒理学]