氧化石墨烯促进NIH3T3细胞在石墨烯/明胶纳米膜上的增殖与粘附的实验研究  

Experimental study on the proliferation and adhesion of NIH3T3 cells on graphene/gelatin nanofilm

作　　者：孙岩峰[1] 刘伟 沈远 杨皛宁 朱惠敏 马金旭 周瑾[2] SUN Yanfeng;LIU Wei;SHEN Yuan;YANG Xiaoning;ZHU Huimin;MA Jinxu;ZHOU Jin(Academy of Military Medical,Institute of Basic Medical Sciences,Department of Advanced Interdisciplinary Studies,Beijing 100080,China;The armed police general hospital,Pediatric,Clinical Cancer,Beijing 100080)

机构地区：[1]武警总医院儿科临床肿瘤,北京100080 [2]军事医学科学院基础医学研究所前沿交叉学科研究室,北京100080

出　　处：《生物医学工程研究》2018年第1期56-60,76,共6页Journal Of Biomedical Engineering Research

基　　金：国家自然科学基金资助项目(31370987,81622027)

摘　　要：本研究旨在探索氧化石墨烯/明胶(graphene oxide/gelatin)薄膜材料对小鼠胚胎成纤维细胞(NIH3T3)活性、增殖与粘附能力的影响规律。利用扫描电镜观察材料表面微观结构。活-死细胞染色检测氧化石墨烯/明胶薄膜材料上的NIH3T3细胞活性,CCK-8法进行细胞增殖评价。利用免疫荧光、Westernblot检测Vimentin表达,采用实时定量RT-PCR检测粘附相关基因α-actin、ColⅠ、ColⅢ、Vinculin、Vimentin的mRNA表达。添加氧化石墨烯后材料表面形成明显的褶皱结构。与对照组相比,实验组的成纤维细胞活性良好,具有更强的增殖能力,且高表达α-actin、ColⅠ、ColⅢ、及Vinculin。氧化石墨烯/明胶薄膜材料促进NIH3T3细胞的增殖与粘附。To explore the effect of Graphene oxide/gelatin(GO/gelatin)on the growth and adhesion of mouse embryonic fibroblasts(NIH3T3).The microstructure of the material was examined by scanning electron microscopy.NIH3T3 cells were inoculated on the graphene/gelatin membrane.Cell proliferation was detected by CCK-8,and the cell viability was observed by live-dead staining.The expression of Vimentin was detected by immunofluorescence and W estern blotting,the mRNA expression levels of adhesion-related genes a-actin,Col I,ColⅢ,and Vinculin were detected by real-time quantitative RT-PCR.The results showed that after adding graphene oxide,the surface of the material becam rough,forming a more obvious fold structure.The results of CCK-8 detection showed that the fibroblasts grew on the graphene-enriched material had good activity and had stronger ability to proliferate compared with the gelatin membrane after the third day.NIH3T3 cells on the graphene/gelatin membrane expressed adhesion genes such as a-actin,Col I,ColⅢ,and Vinculin highly.Oxidized graphene/gelatin material promotes the proliferation and adhesion of NIH3T3 cells

关 键 词：氧化石墨稀 NIH3T3 生物相容性 粘附 

分 类 号：R318[医药卫生—生物医学工程]