栅藻作为生物指示剂的生物延迟发光研究  

The biological delayed luminescence research of Scenedesmus as biological indicator

作　　者：张玉风[1,2] 杨美娜[2] 庞靖祥[2] 周宝宸 韩金祥[2] ZHANG Yufeng;YANG Meina;PANG Jingxiang;ZHOU Baochen;HAN Jinxiang(School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250020,China;Shandong Academy of Medical Sciences,Shandong Medical Biotechnological Center,Jinan 250062)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250020 [2]山东省医学科学院医药生物技术研究中心,山东济南250062

出　　处：《生物医学工程研究》2018年第1期116-120,共5页Journal Of Biomedical Engineering Research

基　　金：国家国际科技合作专项资助(2014DFA30380);国家自然科学基金资助项目(81273997);山东省中医药科技发展计划项目(2013-215);山东省医学科学院医药卫生科技创新工程

摘　　要：通过测量不同实验条件下栅藻的延迟发光动力学行为,确定最优实验条件,为栅藻作为生物指示剂奠定基础。用YPMS-2生物光子测量仪检测栅藻延迟发光,将测得的数据用Statistica 10.0软件进行拟合、处理,得到表征栅藻性质的可靠参数斜率k。栅藻的最佳使用浓度为2.5~3.5×107个/m L,栅藻使用的最佳生长阶段为40~60 d,加入不同中药煎煮液的栅藻斜率k值有较大差异,为研究中药药性提供了新的方向。By measuring the delayed luminescence dynamics behavior of Scenedesmus in different experimental conditions,to determine the optimal experimental conditions,and lay a foundation for Scenedesmus as biological indicator.The delayed luminescence of Scenedesmus was detected with YPMS-2 biophoton measuring instrument.The measured data were fitted and analyzed with statistica 10.0 software.Slope k,a reliable parameter that characterizes the nature of Scenedesmus is obtained.The optimal concentration range is(2.5~3.5)x l0^7个/mL个/mL of using Scenedesmus.Growth of 40~60 d Scenedesmus is most suitable for use.The slope k value of Scenedesmus after adding different herbal decoction liquid has bigger differences.It provides a new direction for the study of Chinese medicinal properties.

关 键 词：栅藻 生物光子 延迟发光 生物指示剂 条件优化 中药 

分 类 号：R318[医药卫生—生物医学工程]