miR-142-3p在结直肠癌组织与细胞中的表达及对细胞增殖影响研究  

The expression of miR-142-3p in colorectal cancer and its effect on proliferation

作　　者：朱文侠[1] 杨玲[1] 成钧[1] 杨彦玲[1] 韩振奎[1] 韩继明[1] 黄广智[1] 王航辉 Zhu Wenxia;Yang Ling;Cheng Jun(Medical College of Yan’an University,Yan’an 71600)

机构地区：[1]延安大学医学院,延安716000 [2]西安交通大学附属红会医院,西安710054

出　　处：《陕西医学杂志》2018年第3期282-286,共5页Shaanxi Medical Journal

基　　金：陕西省教育厅科学研究计划项目(2013JK0754);中国博士后科研项目(2016M600804);陕西省博士后科研项目(2016BSHEDZZ92);西安市卫生和计划生育委员会科研项目(J201602023);西安市红会医院科研项目(YJ2014008);西安市科技局科研项目[2017115FS/YX009(16)]

摘　　要：目的:观察微小RNA-142-3p在结直肠癌组织与细胞中的表达,及其对人结直肠癌细胞增殖的影响。方法:通过组织原位杂交的方法,检测miR142-3p在结直肠癌患者组织中的表达。通过Realtime-PCR检测结直肠癌细胞系中的miR142-3p表达。构建pSliencer 4.1-CMV-miR142-3p过表达载体,并转染结直肠癌细胞Caco2,通过Realtime PCR检测重组质粒pSilencer4.1-CMVmiR142-3p载体在结直肠癌细胞系的表达情况。通过Western Blot检测细胞周期相关蛋白CyclinD1的表达变化。结果:miR142-3p主要定位于细胞的胞浆,在癌组织和癌旁组织中miR142-3p的表达存在差异。miR142-3p结直肠癌细胞系中呈低表达。在结直肠癌细胞系中过表达miR142-3p可下调细胞周期相关蛋白CyclinD1,抑制细胞增殖。结论:miR142-3p抑制结直肠癌Caco2细胞的增殖,下调细胞周期相关蛋白CyclinD1,有望成为结直肠癌诊断和治疗的潜在靶点。Objective:To observe the expression of microRNA-142-3p in colorectal cancer and its effect on the proliferation of human colorectal cancer cells.Methods:The expression of miR142-3p in the tissues of colorectal cancer was detected by in situ hybridization.The expression of miR142-3p in colorectal cancer cell lines was detected by Realtime-PCR.Construction of pSliencer 4.1-CMV-miR142-3p overexpressing vector,the colorectal cancer cells Caco2 was transfected.The expression of recombinant plasmid pSilencer4.1-CMV-miR142-3p vector in colorectal cancer cell lines was detected by Realtime PCR.The expression of CyclinD1 was detected by Western Blot.Results:miR142-3p was mainly located in the cytoplasm of cells,The expression of miR142-3p was different in cancer tissues and adjacent tissues.The expression of miR142-3p in colorectal cancer cell lines was low.Overexpression of miR142-3p in colorectal cancer cell lines may down-regulate the expression of cell cycle-associated protein CyclinD1,which inhibit cell proliferation.Conclusion:miR142-3p can inhibits the proliferation of colorectal cancer CAGE2 cells and down-regulation the expression of CyclinD1 and is expected to be a potential target for the diagnosis and treatment of colorectal cancer.

关 键 词：结直肠肿瘤/病理生理学 ＠微小分子142-3p 增殖 

分 类 号：R392.3[医药卫生—免疫学]