不同结构羊舍空气可培养真菌ERIC-PCR指纹图谱分析  

ERIC-PCR Fingerprinting Analysis of Airborne Fungi in Sheep Sheds with Different Structures

作　　者：乔婷婷[1] 于欢[1] 段晋伟 刘家祺[1] 白燕雨 郭耀[1] 高晓莎 张艳冰 霍乃蕊[1] 刘兴国[2] 古少鹏[1] Qiao Tingting;Yu Huan;Duan Jinwei;Liu Jiaqi;Bai Yanyu;Guo Yao;Gao Xiaosha;Zhang Yanbing;Huo Nairui;Liu Xingguo;Gu Shaopeng(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu,Shanxi 030801,China;China Animal Disease Prevention and Control Center,Beijing 100125)

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]中国动物疫病预防控制中心,北京100125

出　　处：《中国动物检疫》2018年第4期106-110,共5页China Animal Health Inspection

基　　金：国家自然基金(31201437);中国动物疫病预防控制中心横向课题(2013HX45);山西省农业厅横向课题(2013HX35)

摘　　要：为了解圈舍空气真菌菌群结构,为羊舍选择及环境控制提供依据,应用AGI-30液体空气采样器,以超纯水为采样基质,采集地面平养和高床平养2种结构羊舍的气载真菌,经马铃薯葡萄糖肉汤富集培养后提取DNA,进行ERIC-PCR指纹图谱分析。结果显示:2种结构羊舍的气载真菌菌群结构存在差异,但有一些相同的优势菌群;高床平养羊舍的多样性指数高于地面羊舍,但优势度指数偏低;相似性聚类分析显示,相同结构羊舍同一季节气载真菌菌群结构相对稳定,不同季节之间真菌菌群结构变化较大。结果表明,羊舍内气载真菌菌群结构变化较大,且受羊舍结构和季节的影响。In order to investigate the structure of airborne fungi in sheep sheds,so as to provide basis for sheep shed selection and environment control.AGI-30 sampler was used to collect the ultrapure water as the medium,and airborne fungi from 2 kinds of sheep sheds(including structures of ground raising and high-bed raising)were collected.After enrichment cultivation with potato dextrose broth,the fungal genomic DNA was extracted and the diversity of airborne fungi was analyzed by ERIC-PCR.The results showed that there were differences in the structure of airborne fungal flora in the two kinds of sheep sheds,and there were some similar dominant bacteria flora.The diversity index in the sheds with high beds was higher than that of the ground sheep sheds,but the dominance index was low.Similarity clustering analysis showed that the structure of airborne fungi in the sheds with same structure was stable in the same season,and it changed greatly in different seasons.As a conclusion,the diversity of airborne fungi was variable in sheep sheds and was affected by both sheep shed structures and seasons.

关 键 词：羊舍 结构 可培养气载真菌 ERIC-PCR 

分 类 号：S851.24[农业科学—预防兽医学]