基于灯盏花素单抗的Ic-ELISA快速检测方法建立  

Rapid Detection of Breviscapine Monoclonal Antibody Based on Indirect Competitive ELISA

作　　者：刘玉龙 吴华 徐宏宇[2] LIU Yu-long;WU Hua;XU Hong-yu(Department of pharmacy,Binhu hospital,Hefei Anhui 23000,China;Department of pharmacy of the Fourth Military Medical University,Xian Shanxi 710032,China)

机构地区：[1]安徽省合肥市滨湖医院药剂科,安徽合肥23000 [2]中国人民解放军空军军医大学药学系,陕西西安710032

出　　处：《药品评价》2018年第6期40-44,共5页Drug Evaluation

摘　　要：目的:建立一种间接竞争ELISA法检测灯盏花素。方法:抗原包被于固相载体,加入灯盏花素单克隆抗体,酶标二抗建立预先体系,然后用间接竞争ELISA法检测待检抗原,评价此法的竞争抑制率、灵敏度、特异性、精密度等参数,并实际测量样品。结果:成功建立基于灯盏花素单克隆抗体间接竞争ELISA检测法,灵敏度为15ng/m L,与结构类似物交叉反应率均小于0.5%,板内精密度0.82%~1.37%,板间精密度2.45%~5.64%,平均回收率为101.59%。结论:本研究建立的Ic-ELISA法简便可靠,可用于药材、药物、药品中灯盏花素的检测,为研制标准商用试剂盒提供了研究基础,具有广阔的应用前景。Objective:To establish an indirect competitive ELISA method for the determination of breviscapine.Methods:Antigen was coated on the solid phase carrier,added Breviscapine monoclonal antibody,and enzyme-labeled second antibody to establish the pre-system.Indirect competition ELISA test was used to detect antigen and the competitive inhibition rate,sensitivity,specificity and precision of this method were evaluated,and the samples were measured.Results:Indirect competitive ELISA method was established.The sensitivity was 15ng/ml;cross-reactivity rate with other structural analogues was less than 0.5%accuracy within 0.82%~1.37%;the plate,inter-plate precision was 2.45%~5.64%.The average recovery was 101.59%.Conclusion:The ic-ELISA method established in this study can be used for the detection of breviscapine in herbs,medicines and pharmaceuticals.It provides a basis for the development of kits,and has broad application prospects.

关 键 词：灯盏花素 间接竞争 ELISA 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]