结球甘蓝小孢子培养条件优化及高代自交系胚状体诱导研究  

Studies on Optimization of Cabbage Isolated Microspore Culture Conditions and Generation of Embryoids from High-generation Inbred Lines

作　　者：苏贺楠 韩风庆 杨丽梅[1] 庄木[1] 张扬勇[1] 王勇 李占省[1] 方智远[1] 吕红豪[1] SU He-nan;HAN Feng-qing;YANG Li-mei;ZHUANG Mu;ZHANG Yang-yong;WANG Yong;LI Zhan-sheng;FANG Zhi-yuan;LYU Hong-hao(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京100081

出　　处：《中国蔬菜》2018年第4期30-36,共7页China Vegetables

基　　金：国家重点研发计划项目(2017YFD0101804;2016YFD0100204);中国农业科学院科技创新工程项目(CAASASTIP-IVFCAAS);大宗蔬菜产业技术体系项目(nycytx-35-gw01)

摘　　要：以25份不同类型的结球甘蓝为试验材料,研究游离小孢子培养过程中影响胚状体产生的因素并进行了优化;同时对结球甘蓝高代自交系的诱导出胚情况进行了研究。结果表明:基因型是影响结球甘蓝胚状体产生的关键因素,参试材料中有11份(2份一代杂种和9份高代自交系)出胚,其中一代杂种中中甘628出胚率(19.8个·蕾^(-1))最高,高代自交系中01-88出胚率(47.5个·蕾^(-1))最高,极显著高于其他参试材料;花蕾长度3.0~3.5 mm、花药长度∶花瓣长度为3∶2至2∶1时,处于单核靠边期的小孢子比例最高,是适合小孢子培养的最佳取样时期;初花期和盛花期是理想的取蕾时期;添加适量活性炭可以极显著提高胚诱导率。Taking 25 different types of cabbage(Brassica oleracea L.var.capitata L.)accessions as material,this paper studied on the factors affecting the generation of embryoids in culturing isolated microspore and conducted optimization.The embryogenesis for high-generation inbred lines was studied as well.The results showed that the donor plant genotype is the key factor affecting embryogenesis.Embryoids were induced from 11 accessions among all the tested materials.The highest embryoid inducing rate was 19.8 per bud in the hybrid‘Zhonggan 628’and 47.5 per bud in inbred line‘01-88’,which were extremely significantly higher than that of the other accessions.Floral buds with anther length between 3.0-3.5 mm,and ratio of anther length to petal length of 3∶2-2∶1,could produce the highest proportion of microspores at late-uninucleate stage.Early and florescence stages were the optimum sampling periods.Adding active carbon could extremely significantly improve embryoid induction rate.

关 键 词：结球甘蓝 游离小孢子培养 条件优化 高代自交系 胚状体诱导 

分 类 号：S635.1[农业科学—蔬菜学]